从伊朗中部儿科患者腹泻样本中分离出的频率。
Frequency of Isolated from Diarrhea Samples of Pediatric Patients at Central Iran.
作者信息
Abbasi Elnaz, Amouzandeh-Nobaveh Alireza, Ghaznavi-Rad Ehsanollah
机构信息
Department of Microbiology & Immunology, Faculty of Medicine, Arak University of Medical Sciences.
Department of Microbiology & Immunology, Khomein University of Medical Sciences, Khomein, Iran.
出版信息
Rep Biochem Mol Biol. 2019 Jul;8(2):172-177.
BACKGROUND
is a primarily foodborne bacterial pathogen that is one of the causative agents of gastroenteritis. However, the prevalence of infection in pediatric patients with diarrheal disease is not clearly identified in the Iranian population. This study aimed to investigate the frequency of isolates found in infectious diarrhea samples of pediatric patients in an Iranian population.
METHODS
A total of 173 infectious diarrhea samples collected from pediatric patients were used in this crosssectional study. Samples were collected from patients referred to the Children's Educational-Therapeutic Center affiliated with the Arak University of Medical Sciences in Arak, Iran from May-September 2015. To identify the presence of , the samples were directly inoculated into the Listeria Enrichment Broth Base through cold enrichment, then plated onto isolated exclusive Listeria Selective Agar Base. As an alternative method for identifying , Polymerase Chain Reaction (PCR) of the gene was used.
RESULTS
Of the 173 infectious diarrhea samples, eight (4.6%) with were identified using exclusive culture media, while nine (5.2%) were identified using PCR. The majority of infections (seven cases (77.7%)) were observed in children under the age of five.
CONCLUSION
Our results show infections to have a low prevalence for causing diarrhea in children in the central region of Iran. This should be taken into consideration by pediatricians when treating intestinal diseases.
背景
是一种主要通过食物传播的细菌病原体，是肠胃炎的致病因子之一。然而，伊朗人群中腹泻病患儿感染的患病率尚未明确确定。本研究旨在调查伊朗人群中儿童感染性腹泻样本中分离株的频率。
方法
本横断面研究共使用了从儿科患者收集的173份感染性腹泻样本。样本于2015年5月至9月从转诊至伊朗阿拉克医科大学附属儿童教育治疗中心的患者中采集。为了鉴定的存在，样本通过冷增菌直接接种到李斯特菌增菌肉汤基础培养基中，然后接种到分离的专用李斯特菌选择性琼脂基础培养基上。作为鉴定的替代方法，使用基因的聚合酶链反应（PCR）。
结果
在173份感染性腹泻样本中，使用专用培养基鉴定出8份（4.6%）感染，而使用PCR鉴定出9份（5.2%）。大多数感染（7例（77.7%））发生在5岁以下儿童中。
结论
我们的结果表明，在伊朗中部地区，感染导致儿童腹泻的患病率较低。儿科医生在治疗肠道疾病时应考虑到这一点。
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