Abbasi Elnaz, Ghaznavi-Rad Ehsanollah
Department of Microbiology and Immunology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Islamic Republic of Iran.
Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Islamic Republic of Iran.
BMC Gastroenterol. 2021 Mar 30;21(1):140. doi: 10.1186/s12876-021-01719-3.
This study aimed to investigate the frequency and the antibiotic resistance patterns of Salmonella species that were isolated from infectious diarrhea samples taken from pediatric patients in central Iran.
The study analyzed 230 stool specimens that were cultured on XLD, MacConkey agar and GN broth. Polymerase chain reaction (PCR) assay was used to identify the Salmonella genus. The antibiotic resistance profiles and the frequency of quinolone and integron genes were obtained.
Out of 230 samples of infectious diarrhea, 21 (9.1%) cases of Salmonella spp. were identified using culture methods. Another 28 (12.1%) samples had positive PCR results, with S. serovar Paratyphi B and C (9/21; 42.8%) and S. Typhi (3/21; 14.3%) being the most recognized. The highest antibiotic resistance rates were found for nalidixic acid (15/21; 71.4%), tetracycline (9/21; 42.8%). However, six (28.5%) of isolates were found resistant to cotrimoxazole, ampicillin and chloramphenicol. Among the plasmid-mediated quinolone resistance (PMQR) determinants, qnrS, qnrA, and qnrB were positive in (9/15; 60%), (6/15; 40%) and (3/15; 20%) of the isolates, respectively. Class 1 and 2 integrons were identified in 15 (71.4%) and 3 (14.3%) isolates, respectively.
High rates of quinolone resistant and low frequency of MDR Salmonella spp. isolates were identified in central Iran, similar to findings in other parts of Asia. To prevent the spread of these resistant strains, the antimicrobial resistance of Salmonella spp. isolates should be under constant surveillance, and empiric antibiotic therapy should be adapted appropriately.
本研究旨在调查从伊朗中部儿科患者感染性腹泻样本中分离出的沙门氏菌属的频率及其抗生素耐药模式。
该研究分析了230份在XLD、麦康凯琼脂和GN肉汤上培养的粪便标本。采用聚合酶链反应（PCR）检测法鉴定沙门氏菌属。获得了抗生素耐药谱以及喹诺酮和整合子基因的频率。
在230份感染性腹泻样本中，采用培养方法鉴定出21例（9.1%）沙门氏菌属病例。另外28份（12.1%）样本PCR结果呈阳性，其中副伤寒乙和丙沙门氏菌（9/21；42.8%）和伤寒沙门氏菌（3/21；14.3%）最为常见。萘啶酸（15/21；71.4%）、四环素（9/21；42.8%）的抗生素耐药率最高。然而，6株（28.5%）分离株对复方新诺明、氨苄西林和氯霉素耐药。在质粒介导的喹诺酮耐药（PMQR）决定簇中，qnrS、qnrA和qnrB在分离株中的阳性率分别为（9/15；60%）、（6/15；40%）和（3/15；20%）。分别在15株（71.4%）和3株（14.3%）分离株中鉴定出1类和2类整合子。
在伊朗中部地区，沙门氏菌属分离株的喹诺酮耐药率较高，多重耐药率较低，这与亚洲其他地区的研究结果相似。为防止这些耐药菌株的传播，应持续监测沙门氏菌属分离株的抗菌药物耐药性，并适当调整经验性抗生素治疗方案。
