Institute of Zoology, Bahauddin Zakariya University, Multan 60800, Pakistan.
Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Manouba 2011, Tunisia.
Genes (Basel). 2022 Dec 1;13(12):2262. doi: 10.3390/genes13122262.
Despite extensive industrial use, the biocompatibility of nanocomposites has not been extensively explored. The present study was designed to report the effect of variable doses of a newly synthesized nanocomposite, Neodymium Zirconate Zinc Sulfide, on selective serum and complete blood count parameters and on the oxidative stress markers from the vital organs of albino mice. Albino mice (C57BL/6 strain, 5 weeks old) of both sexes were orally treated for 11 days, either with 10 mg (low dose) or 20 mg/mL saline/kg body weight (high dose) of Neodymium Zirconate Zinc Sulfide nanocomposite. A control group that was not treated with the nanocomposite but with saline solution was also maintained. Data analysis revealed that high-dose nanocomposite-treated male mice had significantly reduced hemoglobin concentration as compared to the control males. Female mice treated with both doses of nanocomposite had higher serum triglyceride levels than controls. High-dose-treated female mice had elevated serum cholesterol concentration compared to their saline-treated controls. Oxidative stress marker analysis from selected organs indicated that concentrations of malonaldehyde (MDA) in the kidney and liver, Superoxide dismutase (SOD) levels in the brain and catalase in the kidney of male mice treated with the nanocomposite were significantly higher than in the control group, whereas SOD in the heart, MDA in the heart and kidney and catalase levels in the kidney were significantly disrupted in female mice compared to their respective controls.
尽管纳米复合材料已经得到广泛应用，但人们对其生物相容性的研究还不够充分。本研究旨在报告一种新合成的纳米复合材料——钕锆酸锌硫化物，不同剂量对雄性和雌性白化小鼠的选择性血清和全血细胞计数参数以及重要器官氧化应激标志物的影响。将 C57BL/6 品系的雄性和雌性白化小鼠（5 周龄）分别用 10mg（低剂量）或 20mg/mL 生理盐水/kg 体重（高剂量）的钕锆酸锌硫化物纳米复合材料进行口服处理，连续处理 11 天。同时还设立了一个未用纳米复合材料处理、但用生理盐水处理的对照组。数据分析显示，与对照组雄性相比，高剂量纳米复合材料处理的雄性小鼠的血红蛋白浓度显著降低。与对照组相比，接受两种剂量纳米复合材料处理的雌性小鼠的血清甘油三酯水平更高。与生理盐水处理的对照组相比，高剂量处理的雌性小鼠的血清胆固醇浓度升高。对选定器官的氧化应激标志物分析表明，与对照组相比，接受纳米复合材料处理的雄性小鼠的肾脏和肝脏中的丙二醛（MDA）浓度、大脑中的超氧化物歧化酶（SOD）水平和肾脏中的过氧化氢酶活性均显著升高，而心脏中的 SOD、心脏和肾脏中的 MDA 以及肾脏中的过氧化氢酶活性在雌性小鼠中显著受到破坏。
