Department of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 Milan, Italy; Veterinary Teaching Hospital, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
Department of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 Milan, Italy; Veterinary Teaching Hospital, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
Vet J. 2019 Dec;254:105358. doi: 10.1016/j.tvjl.2019.105358. Epub 2019 Aug 22.
Serum homocysteine (Hcy) increases in people and dogs with chronic kidney disease (CKD). Hyperhomocysteinemia (HHcy) has also been associated with CKD-related hypertension and proteinuria. The aims of this study were to: (1) validate an enzymatic method for quantification of Hcy in feline serum; (2) evaluate whether HHcy was associated with the presence and severity of CKD, proteinuria or hypertension; and (3) determine whether HHcy could predict disease progression. The intra- and inter-assay coefficients of variation (CVs) and the recovery rates of linearity under dilution and spiking recovery tests of the enzymatic method were 3.1-6.7%, 11.6-12.5%, 96.9±5.4% and 96.9±5.4%, respectively. Healthy cats at risk of CKD (n=17) and cats with CKD (n=19) were sampled over a 6-month period (63 samples in total). Cats with CKD had significantly higher Hcy concentrations (P=0.005) than cats at risk. The concentration of Hcy was higher (P=0.002) in moderate-severe CKD than in mild CKD and correlated moderately with serum creatinine (P<0.0001; r=0.51). The concentration of Hcy increased with the magnitude of proteinuria and correlated weakly with urinary protein to creatinine ratio (P=0.045; r=0.26). HHcy was not associated with hypertension. At the time of enrollment, Hcy concentration was significantly higher (P=0.046) in cats that developed CKD compared to cats that remained stable. The enzymatic method for Hcy measurement in feline serum was precise and accurate. HHcy was relatively common in cats with advanced CKD and seemed to predict disease progression, but further studies are warranted.
血清同型半胱氨酸（Hcy）在患有慢性肾脏病（CKD）的人和犬中增加。高同型半胱氨酸血症（HHcy）也与 CKD 相关的高血压和蛋白尿有关。本研究的目的是：（1）验证用于检测猫血清中 Hcy 的酶法；（2）评估 HHcy 是否与 CKD、蛋白尿或高血压的存在和严重程度相关；（3）确定 HHcy 是否可以预测疾病进展。该酶法的批内和批间变异系数（CV）以及稀释和加标回收线性度的回收率分别为 3.1-6.7%、11.6-12.5%、96.9±5.4%和 96.9±5.4%。患有 CKD 风险的健康猫（n=17）和患有 CKD 的猫（n=19）在 6 个月的时间内进行了采样（共 63 个样本）。患有 CKD 的猫的 Hcy 浓度明显高于有风险的猫（P=0.005）。中度-重度 CKD 的 Hcy 浓度高于轻度 CKD（P=0.002），并与血清肌酐中度相关（P<0.0001；r=0.51）。Hcy 浓度随蛋白尿程度的增加而升高，与尿蛋白/肌酐比值弱相关（P=0.045；r=0.26）。HHcy 与高血压无关。在入组时，与病情稳定的猫相比，发生 CKD 的猫的 Hcy 浓度明显更高（P=0.046）。用于检测猫血清中 Hcy 的酶法精确而准确。HHcy 在患有晚期 CKD 的猫中相对常见，似乎可以预测疾病进展，但需要进一步研究。
