• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

卵巢组织和整个卵巢的冷冻保存技术

Techniques of Cryopreservation for Ovarian Tissue and Whole Ovary.

作者信息

Arav Amir, Patrizio Pasquale

机构信息

FertileSafe Ltd, Ness Ziona, Israel.

Yale Fertility Center, Yale University, New Haven, CT, USA.

出版信息

Clin Med Insights Reprod Health. 2019 Dec 3;13:1179558119884945. doi: 10.1177/1179558119884945. eCollection 2019.

DOI:10.1177/1179558119884945
PMID:31839716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6893924/
Abstract

Cryopreservation of ovarian tissue has been considered experimental for many years, but very recently the American Society of Reproductive Medicine is reviewing the process and perhaps soon will remove the label of "experimental" and recognize it as an established method for preserving female fertility when gonadotoxic treatments cannot be delayed or in patients before puberty or when there is desire to cryopreserve more than just few oocytes. This article discusses in detail the 3 methodologies used for cryopreservation: (a) slow freezing, (b) directional freezing, and (c) vitrification.

摘要

多年来，卵巢组织冷冻保存一直被视为实验性技术，但最近美国生殖医学学会正在审查这一技术流程，或许很快就会取消“实验性”这一标签，并将其认可为一种成熟的方法，用于在性腺毒性治疗无法推迟时、青春期前患者中或当人们希望冷冻保存的不止是少量卵母细胞时保存女性生育力。本文详细讨论了用于冷冻保存的三种方法：（a）慢速冷冻，（b）定向冷冻，以及（c）玻璃化冷冻。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebfe/6893924/2dbf71a8e9c7/10.1177_1179558119884945-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebfe/6893924/245c28ef9ee8/10.1177_1179558119884945-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebfe/6893924/f8e04dc35d48/10.1177_1179558119884945-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebfe/6893924/2dbf71a8e9c7/10.1177_1179558119884945-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebfe/6893924/245c28ef9ee8/10.1177_1179558119884945-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebfe/6893924/f8e04dc35d48/10.1177_1179558119884945-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebfe/6893924/2dbf71a8e9c7/10.1177_1179558119884945-fig3.jpg

相似文献

1
Techniques of Cryopreservation for Ovarian Tissue and Whole Ovary.卵巢组织和整个卵巢的冷冻保存技术
Clin Med Insights Reprod Health. 2019 Dec 3;13:1179558119884945. doi: 10.1177/1179558119884945. eCollection 2019.
2
Safeguarding Fertility With Whole Ovary Cryopreservation and Microvascular Transplantation: Higher Follicular Survival With Vitrification Than With Slow Freezing in a Ewe Model.通过全卵巢冷冻保存和微血管移植保护生育能力：在母羊模型中，玻璃化冷冻比慢速冷冻具有更高的卵泡存活率。
Transplantation. 2016 Sep;100(9):1889-97. doi: 10.1097/TP.0000000000001296.
3
Cryobiology in human assisted reproductive technology. Would Hippocrates approve?人类辅助生殖技术中的低温生物学。希波克拉底会赞同吗？
Early Pregnancy (Cherry Hill). 2001 Jul;5(3):211-3.
4
Directional freezing: a solution to the methodological challenges to preserve large organs.定向冷冻：解决保存大型器官方法学挑战的一种方案。
Semin Reprod Med. 2009 Nov;27(6):438-42. doi: 10.1055/s-0029-1241052. Epub 2009 Oct 5.
5
Cryopreservation of in vitro matured oocytes in addition to ovarian tissue freezing for fertility preservation in paediatric female cancer patients before and after cancer therapy.在癌症治疗前后，对儿科女性癌症患者进行体外成熟卵母细胞的冷冻保存以及卵巢组织冷冻，以保留生育能力。
Hum Reprod. 2016 Apr;31(4):750-62. doi: 10.1093/humrep/dew007. Epub 2016 Feb 4.
6
Chapter 16 Directional Freezing of Ovarian Tissue and Freeze-Drying of Stem Cells for Fertility Preservation.第16章 用于生育力保存的卵巢组织定向冷冻和干细胞冻干
Methods Mol Biol. 2017;1568:223-229. doi: 10.1007/978-1-4939-6828-2_16.
7
Novel needle immersed vitrification: a practical and convenient method with potential advantages in mouse and human ovarian tissue cryopreservation.新型针式浸入式玻璃化冷冻法：一种在小鼠和人类卵巢组织冷冻保存方面具有潜在优势的实用便捷方法。
Hum Reprod. 2008 Oct;23(10):2256-65. doi: 10.1093/humrep/den255. Epub 2008 Jul 9.
8
Oocyte vitrification versus ovarian cortex transplantation in fertility preservation for adult women undergoing gonadotoxic treatments: a prospective cohort study.卵母细胞玻璃化与卵巢皮质移植在接受性腺毒性治疗的成年女性生育力保存中的比较：一项前瞻性队列研究。
Fertil Steril. 2018 Mar;109(3):478-485.e2. doi: 10.1016/j.fertnstert.2017.11.018. Epub 2018 Feb 7.
9
Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue.人类卵巢组织冷冻保存中玻璃化冷冻与程序降温冷冻的比较
Hum Reprod. 2009 Jul;24(7):1670-83. doi: 10.1093/humrep/dep079. Epub 2009 Apr 9.
10
Ovarian tissue vitrification is more efficient than slow freezing in protecting oocyte and granulosa cell DNA integrity.在保护卵母细胞和颗粒细胞DNA完整性方面，卵巢组织玻璃化冷冻比慢速冷冻更有效。
Syst Biol Reprod Med. 2014 Dec;60(6):317-22. doi: 10.3109/19396368.2014.923542. Epub 2014 Jun 4.

引用本文的文献

1
Assessing the knowledge, attitudes, and perceptions of ovarian cryopreservation technology among women with ovarian diseases.评估卵巢疾病女性对卵巢冷冻保存技术的知识、态度和认知。
Front Public Health. 2025 Jun 23;13:1612492. doi: 10.3389/fpubh.2025.1612492. eCollection 2025.
2
Current State and Challenges of Tissue and Organ Cryopreservation in Biobanking.生物银行组织和器官低温保存的现状和挑战。
Int J Mol Sci. 2024 Oct 16;25(20):11124. doi: 10.3390/ijms252011124.
3
Thiol-yne click crosslink hyaluronic acid/chitosan hydrogel for three-dimensional follicle development.

本文引用的文献

1
A new, simple, automatic vitrification device: preliminary results with murine and bovine oocytes and embryos.一种新的、简单的、自动的玻璃化装置：与小鼠和牛卵母细胞和胚胎的初步结果。
J Assist Reprod Genet. 2018 Jul;35(7):1161-1168. doi: 10.1007/s10815-018-1210-9. Epub 2018 May 25.
2
Oocyte vitrification versus ovarian cortex transplantation in fertility preservation for adult women undergoing gonadotoxic treatments: a prospective cohort study.卵母细胞玻璃化与卵巢皮质移植在接受性腺毒性治疗的成年女性生育力保存中的比较：一项前瞻性队列研究。
Fertil Steril. 2018 Mar;109(3):478-485.e2. doi: 10.1016/j.fertnstert.2017.11.018. Epub 2018 Feb 7.
3
用于三维卵泡发育的硫醇-炔点击交联透明质酸/壳聚糖水凝胶
Mater Today Bio. 2023 Nov 17;23:100867. doi: 10.1016/j.mtbio.2023.100867. eCollection 2023 Dec.
4
Cryopreservation of tissues and organs: present, bottlenecks, and future.组织和器官的冷冻保存：现状、瓶颈与未来。
Front Vet Sci. 2023 May 25;10:1201794. doi: 10.3389/fvets.2023.1201794. eCollection 2023.
5
The Late Effects of Cancer Treatment on Female Fertility and the Current Status of Fertility Preservation-A Narrative Review.癌症治疗对女性生育力的晚期影响及生育力保存现状——一项叙述性综述
Life (Basel). 2023 May 17;13(5):1195. doi: 10.3390/life13051195.
6
A comprehensive review and update on human fertility cryopreservation methods and tools.人类生育力冷冻保存方法与工具的全面综述及更新
Front Vet Sci. 2023 Apr 18;10:1151254. doi: 10.3389/fvets.2023.1151254. eCollection 2023.
7
A guide to successful mL to L scale vitrification and rewarming.mL 到 L 规模玻璃化和复温成功指南。
Cryo Letters. 2022 Nov-Dec;43(6):316-321.
8
Fertility preservation strategies for cancerous women: An updated review.癌症女性的生育力保存策略：最新综述。
Turk J Obstet Gynecol. 2022 Jun 27;19(2):152-161. doi: 10.4274/tjod.galenos.2022.42272.
9
Human adipose-derived stromal cells transplantation prolongs reproductive lifespan on mouse models of mild and severe premature ovarian insufficiency.人脂肪来源的基质细胞移植可延长轻度和重度卵巢早衰小鼠模型的生殖寿命。
Stem Cell Res Ther. 2021 Oct 10;12(1):537. doi: 10.1186/s13287-021-02590-5.
10
Hormonal Stimulation of Human Ovarian Xenografts in Mice: Studying Folliculogenesis, Activation, and Oocyte Maturation.在小鼠中进行人卵巢异种移植的激素刺激：研究卵泡发生、激活和卵母细胞成熟。
Endocrinology. 2020 Dec 1;161(12). doi: 10.1210/endocr/bqaa194.
Fertility Preservation in Women.
女性生育力保存
N Engl J Med. 2017 Oct 26;377(17):1657-1665. doi: 10.1056/NEJMra1614676.
4
Fertility preservation for age-related fertility decline.与年龄相关的生育能力下降的生育力保存。
Lancet. 2014 Oct 4;384(9950):1311-9. doi: 10.1016/S0140-6736(14)61261-7.
5
Freeze drying of red blood cells: the use of directional freezing and a new radio frequency lyophilization device.红细胞的冷冻干燥：定向冷冻和新型射频冻干设备的应用。
Biopreserv Biobank. 2012 Aug;10(4):386-94. doi: 10.1089/bio.2012.0021.
6
Fertility preservation in women.女性生育力保存。
Nat Rev Endocrinol. 2013 Dec;9(12):735-49. doi: 10.1038/nrendo.2013.205. Epub 2013 Oct 29.
7
Beneficial effect of directional freezing on in vitro viability of cryopreserved sheep whole ovaries and ovarian cortical slices.定向冻结对冷冻保存绵羊整个卵巢和卵巢皮质切片体外活力的有益影响。
Hum Reprod. 2014 Jan;29(1):114-24. doi: 10.1093/humrep/det377. Epub 2013 Oct 17.
8
Direct comparative analysis of conventional and directional freezing for the cryopreservation of whole ovaries.常规冷冻与定向冷冻在卵巢组织冻存中的直接比较分析。
Fertil Steril. 2013 Oct;100(4):1122-31. doi: 10.1016/j.fertnstert.2013.06.003. Epub 2013 Jul 3.
9
Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue: a review of 60 cases of reimplantation.冷冻卵巢组织移植后卵巢功能和妊娠的恢复：60 例再植入的回顾性研究。
Fertil Steril. 2013 May;99(6):1503-13. doi: 10.1016/j.fertnstert.2013.03.030.
10
Is vitrification of oocytes useful for fertility preservation for age-related fertility decline and in cancer patients?卵母细胞的玻璃化冷冻保存对年龄相关生育力下降和癌症患者的生育力保存有用吗？
Fertil Steril. 2013 May;99(6):1485-95. doi: 10.1016/j.fertnstert.2013.02.050. Epub 2013 Mar 29.