囊性纤维化临床分离株中肠毒素基因簇超抗原的高流行率。
High Prevalence of Enterotoxin Gene Cluster Superantigens in Cystic Fibrosis Clinical Isolates.
机构信息
Stead Family Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA.
Department of Microbiology and Immunology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA.
出版信息
Genes (Basel). 2019 Dec 12;10(12):1036. doi: 10.3390/genes10121036.
BACKGROUND
is a highly prevalent respiratory pathogen in cystic fibrosis (CF). It is unclear how this organism establishes chronic infections in CF airways. We hypothesized that isolates from patients with CF would share common virulence properties that enable chronic infection.
METHODS
77 isolates were obtained from 45 de-identified patients with CF at the University of Iowa. We assessed isolates phenotypically and used genotyping assays to determine the presence or absence of 18 superantigens (SAgs).
RESULTS
We observed phenotypic diversity among isolates from patients with CF. Genotypic analysis for SAgs revealed 79.8% of CF clinical isolates carried all six members of the enterotoxin gene cluster (EGC). MRSA and MSSA isolates had similar prevalence of SAgs. We additionally observed that EGC SAgs were prevalent in isolated from two geographically distinct CF centers.
CONCLUSIONS
SAgs belonging to the EGC are highly prevalent in CF clinical isolates. The greater prevalence in these SAgs in CF airway specimens compared to skin isolates suggests that these toxins confer selective advantage in the CF airway.
背景
是囊性纤维化（CF）中一种高度流行的呼吸道病原体。目前尚不清楚该病原体如何在 CF 气道中建立慢性感染。我们假设来自 CF 患者的 分离株将具有共同的毒力特性，从而实现慢性感染。
方法
从爱荷华大学的 45 名身份不明的 CF 患者中获得了 77 株 分离株。我们对分离株进行了表型评估，并使用基因分型检测确定了 18 种超抗原（SAg）的存在或缺失。
结果
我们观察到来自 CF 患者的 分离株存在表型多样性。对 SAg 的基因分析显示，79.8%的 CF 临床分离株携带肠毒素基因簇（EGC）的所有六个成员。MRSA 和 MSSA 分离株的 SAg 存在率相似。我们还观察到，在来自两个地理位置不同的 CF 中心的 分离株中，EGC SAg 也很普遍。
结论
EGC 属的 SAg 在 CF 临床分离株中高度流行。与皮肤分离株相比，这些毒素在 CF 气道标本中的更高流行率表明，这些毒素在 CF 气道中具有选择性优势。
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