Cheng Yongbiao, Xu Tianbo, Li Sen, Ruan Hailong
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Institute of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Aging (Albany NY). 2019 Dec 16;11(24):12165-12176. doi: 10.18632/aging.102555.
Renal cell carcinoma (RCC) is the most common malignant tumor of the kidney, and its diagnosis and prognosis still lack reliable biomarkers. Glutathione peroxidase 1 (GPX1) has been identified to be highly expressed in a variety of human malignancies. However, few studies have studied the expression of GPX1 and its biological functions in RCC. We attempted to assess the potential of GPX1 as a promising biomarker for RCC diagnosis and prognosis. In this study, we analyzed and explored the public cancer databases (TCGA and ONCOMINE) to conclude that GPX1 is highly expressed in RCC. Meanwhile, we evaluated the expression of GPX1 at the levels of RCC cells and tissues to verify the results of the database. Moreover, high GPX1 levels were positively correlated with short overall survival time, distant metastasis, lymphatic metastasis, and tumor stage. Receiver operating characteristic curve (ROC) analysis showed that high GPX1 levels could distinguish RCC patients from normal subjects (p < 0.0001). Kaplan-Meier curve analysis revealed that high GPX1 levels predicted shorter overall survival time (p = 0.0009). Finally, the functional roles of GPX1 were examined using a GPX1 sh-RNA knockdown method in RCC cell lines. In summary, our results suggest that GPX1 may have the potential to serve as a diagnostic and prognostic biomarker for RCC patients. Moreover, targeting GPX1 may represent as a new therapeutic strategy and direction for RCC patients.
肾细胞癌（RCC）是最常见的肾脏恶性肿瘤，其诊断和预后仍然缺乏可靠的生物标志物。谷胱甘肽过氧化物酶1（GPX1）已被确定在多种人类恶性肿瘤中高表达。然而，很少有研究探讨GPX1在RCC中的表达及其生物学功能。我们试图评估GPX1作为RCC诊断和预后有前景的生物标志物的潜力。在本研究中，我们分析并探索了公共癌症数据库（TCGA和ONCOMINE），得出GPX1在RCC中高表达的结论。同时，我们在RCC细胞和组织水平评估了GPX1的表达，以验证数据库结果。此外，高GPX1水平与总生存时间短、远处转移、淋巴转移和肿瘤分期呈正相关。受试者工作特征曲线（ROC）分析表明，高GPX1水平可区分RCC患者与正常受试者（p < 0.0001）。Kaplan-Meier曲线分析显示，高GPX1水平预示总生存时间较短（p = 0.0009）。最后，在RCC细胞系中使用GPX1 sh-RNA敲低方法检测了GPX1的功能作用。总之，我们的结果表明，GPX1可能有潜力作为RCC患者的诊断和预后生物标志物。此外，靶向GPX1可能代表RCC患者一种新的治疗策略和方向。
