Brzozowa-Zasada Marlena, Piecuch Adam, Bajdak-Rusinek Karolina, Janelt Kamil, Michalski Marek, Klymenko Olesya, Matysiak Natalia
Department of Histology and Cell Pathology in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Department of Medical Genetics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Pharmaceuticals (Basel). 2023 May 12;16(5):740. doi: 10.3390/ph16050740.
Several studies revealed that expression levels of glutathione peroxidase 1 (Gpx-1) can be associated with cancer development, mainly through its role in hydroperoxide scavenging by regulating intracellular reactive oxygen species (ROS) levels. Therefore, our aim was to investigate the expression of Gpx-1 protein in a population of Polish patients with colon adenocarcinoma in the absence of any therapy prior to radical surgery. The study was carried out using colon tissue from patients with adenocarcinoma of the colon confirmed by histopathological examination. Gpx-1 antibody was used to determine the immunohistochemical expression of Gpx-1. The Chitest or Chi test were used to analyse the associations between the immunohistochemical expression of Gpx-1 and clinical parameters. The relationship between Gpx-1 expression, and 5-year patient survival was examined using Kaplan-Meier analysis and the log-rank test. Intracellular localisation of Gpx-1 was detected by the use of transmission electron microscopy (TEM). Western blot analysis was used for the evaluation of Gpx-1 protein expression levels in cancer cell lines in vitro. Immunohistochemical study revealed that the high expression of Gpx-1 was associated with the tumour's histological grade, proliferating cell nuclear antigen (PCNA) immunohistochemical expression, depth of invasion, and angioinvasion (all < 0.001) (4). The high immunohistochemical expression of Gpx-1 is correlated with poor prognosis of colon adenocarcinoma patients.
多项研究表明，谷胱甘肽过氧化物酶1（Gpx-1）的表达水平可能与癌症发展相关，主要是通过其在调节细胞内活性氧（ROS）水平以清除氢过氧化物方面的作用。因此，我们的目的是研究在接受根治性手术前未进行任何治疗的波兰结肠癌患者群体中Gpx-1蛋白的表达情况。该研究使用经组织病理学检查确诊为结肠癌患者的结肠组织进行。使用Gpx-1抗体来确定Gpx-1的免疫组化表达。采用卡方检验或χ²检验分析Gpx-1免疫组化表达与临床参数之间的关联。使用Kaplan-Meier分析和对数秩检验来检查Gpx-1表达与患者5年生存率之间的关系。通过透射电子显微镜（TEM）检测Gpx-1的细胞内定位。蛋白质印迹分析用于评估体外癌细胞系中Gpx-1蛋白的表达水平。免疫组化研究表明，Gpx-1的高表达与肿瘤的组织学分级、增殖细胞核抗原（PCNA）免疫组化表达、浸润深度和血管浸润相关（均P<0.001）（4）。Gpx-1的高免疫组化表达与结肠癌患者的不良预后相关。
