National Institute of Standards and Technology, Material Measurement Laboratory, Chemical Sciences Division, Charleston, South Carolina, 29412, USA.
National Institute of Standards and Technology, Material Measurement Laboratory, Biomolecular Measurement Division, 100 Bureau Drive, Stop 8314, Gaithersburg, Maryland, 2089, USA.
Sci Data. 2019 Dec 18;6(1):324. doi: 10.1038/s41597-019-0336-7.
The National Institute of Standards and Technology (NIST) is creating new, economical, qualitative reference materials and data for proteomics comparisons, benchmarking and harmonization. Here we describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics, a reference material being developed. Consensus identifications using multiple search engines and sample preparations demonstrate a homogeneous and fit-for-purpose material that can be incorporated into automated or manual sample preparation workflows, with the resulting data used to directly assess complete sample-to-data workflows and provide harmonization and benchmarking between laboratories and techniques. Data are available via PRIDE with identifier PXD013608.
美国国家标准与技术研究院 (NIST) 正在为蛋白质组学比较、基准测试和协调创建新的、经济的、定性的参考材料和数据。在这里，我们描述了 RM 8461 人类肝脏蛋白质组学分析的大量数据集，这是正在开发的一种参考材料。使用多个搜索引擎和样品制备进行的共识鉴定表明，这是一种同质的、适用的材料，可以纳入自动化或手动样品制备工作流程，所得数据可直接用于评估完整的样品到数据工作流程，并在实验室和技术之间提供协调和基准测试。数据可通过 PRIDE 标识符 PXD013608 获取。
