Zhu Xuedong, Lin Jen-Wei, Sander Michelle Y
Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.
Neurophotonics Center, Boston University, 24 Cummington Mall, Boston, MA 02215, USA.
Biomed Opt Express. 2019 Nov 27;10(12):6580-6594. doi: 10.1364/BOE.10.006580. eCollection 2019 Dec 1.
The infrared (IR) inhibition of axonal activities in the crayfish neuromuscular preparation is studied using 2 µm IR light pulses with varying durations. The intracellular neuronal activities are monitored with two-electrode current clamp, while the IR-induced temperature changes are measured by the open patch technique simultaneously. It is demonstrated that the IR pulses can reversibly shape or block locally initiated action potentials. Suppression of the AP amplitude and duration and decrease in axonal excitability by IR pulses are quantitatively analyzed. While the AP amplitude and duration decrease similarly during IR illumination, it is discovered that the recovery of the AP duration after the IR pulses is slower than that of the AP amplitude. An IR-induced decrease in the input resistance (8.8%) is detected and discussed together with the temperature dependent changes in channel kinetics as contributing factors for the inhibition reported here.
利用不同持续时间的2微米红外（IR）光脉冲，研究了小龙虾神经肌肉标本中轴突活动的红外抑制作用。采用双电极电流钳监测细胞内神经元活动，同时通过膜片钳技术测量红外诱导的温度变化。结果表明，红外脉冲可以可逆地塑造或阻断局部引发的动作电位。对红外脉冲引起的动作电位幅度和持续时间的抑制以及轴突兴奋性的降低进行了定量分析。虽然在红外照射期间动作电位幅度和持续时间的降低相似，但发现红外脉冲后动作电位持续时间的恢复比动作电位幅度的恢复要慢。检测到红外诱导的输入电阻降低（8.8%），并与通道动力学的温度依赖性变化一起讨论，作为此处报道的抑制作用的促成因素。
