Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17p, 30559 Hannover, Germany.
J Dairy Sci. 2013 Jun;96(6):3959-64. doi: 10.3168/jds.2012-5679. Epub 2013 Mar 30.
Left-sided displacement of the abomasum (LDA) is one of the most common disorders of the digestive system in many dairy breeds and particularly in Holstein dairy cows. We performed a genome-wide association study for 854 German Holstein cows, including 225 cases and 629 controls. All cows were genotyped using the Illumina Bovine SNP50 BeadChip (Illumina Inc., San Diego, CA). After quality control of genotypes, a total of 36,226 informative single nucleotide polymorphisms (SNP) were left for analysis. We used a mixed linear model approach for a genome-wide association study of LDA. In total, 36 SNP located on 17 bovine (Bos taurus) chromosomes (BTA) showed associations with LDA at nominal -log10P-values >3.0. Two of these SNP, located on BTA11 at 46.70 Mb and BTA20 at 16.67 Mb, showed genome-wide significant associations with LDA at -log10P-values >4.6. Pathway analyses indicated genes involved in calcium metabolism and insulin-dependent diabetes mellitus to be factors in the pathogenesis of LDA in German Holstein cows.
左侧腹腔移位（LDA）是许多奶牛品种，尤其是荷斯坦奶牛消化系统最常见的疾病之一。我们对 854 头德国荷斯坦奶牛进行了全基因组关联研究，包括 225 例病例和 629 例对照。所有奶牛均使用 Illumina Bovine SNP50 BeadChip（Illumina Inc.，圣地亚哥，CA）进行基因分型。在对基因型进行质量控制后，共留下了 36,226 个信息性单核苷酸多态性（SNP）进行分析。我们使用混合线性模型方法对 LDA 进行全基因组关联研究。共有 36 个 SNP 位于 17 个牛（Bos taurus）染色体（BTA）上，在名义 -log10P 值>3.0 时与 LDA 相关。其中两个 SNP 位于 BTA11 上的 46.70 Mb 和 BTA20 上的 16.67 Mb，在 -log10P 值>4.6 时与 LDA 具有全基因组显著相关性。途径分析表明，参与钙代谢和胰岛素依赖型糖尿病的基因是德国荷斯坦奶牛 LDA 发病机制的因素。
