Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Selcuklu, Konya, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Selcuk University, Selcuklu, Konya, Turkey.
Vet Q. 2020 Dec;40(1):1-15. doi: 10.1080/01652176.2019.1707907.
Displaced abomasum (DA) is a condition of dairy cows that severely impacts animal welfare and causes huge economic losses. To assess the metabolic status of the disease using metabolomics in serum, urine and liver samples aimed at both water soluble and lipid soluble fractions. Fifty Holstein multiparous cows with DA (42 left, 8 right) and 20 clinically healthy Holstein multiparous cows were used. Left DA was associated with concomitant ketosis in 19 animals and right in two. NMR-based metabolomics approach and hematological and biochemical analyses were performed. Statistical analysis was carried out on H-NMR data after they have been normalized using PQN method. Contrary to generated PCA score plots the OPLS-supervised method revealed differences between healthy animals and diseased ones based on serum water-soluble samples. While water and lipid soluble metabolites decreased in serum samples, fatty acid fractions and cholesterol were increased in liver samples in DA affected cows. The metabolomic and chemical profiles clearly revealed that cows with DA (especially with LDA) were at risk of ketosis and fatty liver. Serum hippuric acid concentration was significantly higher in healthy cows in comparison with LDA, whereas serum glycine concentration was reported higher for healthy when compared to RDA affected animals. A biochemical network and pathway mapping revealed 'valine, leucine and isoleucine biosynthesis' and 'phenylalanine, tyrosine and tryptophan biosynthesis' as the most probable altered metabolic pathway in DA condition. Serum was advocated as the optimal biological matrix for the H-NMR analysis.
奶牛皱胃移位（DA）是一种严重影响动物福利并造成巨大经济损失的疾病。本研究采用代谢组学方法，分别对血清、尿液和肝脏样本的水溶性和脂溶性部分进行分析，旨在评估该疾病的代谢状态。选用 50 头荷斯坦经产奶牛（左 DA42 头，右 DA8 头）和 20 头临床健康的荷斯坦经产奶牛作为研究对象。左 DA 与 19 头动物的酮病有关，右 DA 与 2 头动物的酮病有关。采用基于 NMR 的代谢组学方法以及血液学和生化分析。对使用 PQN 方法归一化后的 H-NMR 数据进行统计分析。与生成的 PCA 得分图相反，基于血清水溶性样本的 OPLS 监督方法揭示了健康动物和患病动物之间的差异。在 DA 患病奶牛中，血清和脂溶性代谢物减少，而肝脏样本中的脂肪酸和胆固醇增加。代谢组学和化学特征清楚地表明，患有 DA（尤其是左 DA）的奶牛有患酮病和脂肪肝的风险。与左 DA 相比，健康奶牛血清中的马尿酸浓度显著升高，而与右 DA 相比，健康奶牛血清中的甘氨酸浓度较高。生化网络和途径映射显示，在 DA 状态下，最有可能改变的代谢途径是“缬氨酸、亮氨酸和异亮氨酸生物合成”和“苯丙氨酸、酪氨酸和色氨酸生物合成”。提倡使用血清作为 H-NMR 分析的最佳生物基质。
