Department of Molecular Genetics and Microbiology, College of Medicine, Emerging Pathogens Institute, 2055 Mowry Road, Gainesville, FL 32610, USA.
J Med Microbiol. 2020 Jan;69(1):139-145. doi: 10.1099/jmm.0.001128. Epub 2019 Dec 18.
Laboratory research with commonly involves the prototype strain PAO1. There is continued concern that PAO1 sublines maintained and propagated in the same laboratory or different laboratories exhibit genetic and phenotypic variability that may affect the reproducibility and validity of research. Whole-genome sequencing and other research identified the locus as a mutational hotspot, but the explication of the diverse mutations present in the various sublines and consequences remained rather cursory. Here we present evidence that MexT sequence diversity is a predictor of PAO1 lineage integrity and define the protein's prototype sequence.
实验室研究通常涉及原型菌株 PAO1。人们一直担心，在同一实验室或不同实验室中保存和传代的 PAO1 亚系表现出遗传和表型的可变性，这可能会影响研究的可重复性和有效性。全基因组测序和其他研究确定了 位点是一个突变热点，但各种亚系中存在的不同突变的解释以及其后果仍然相当粗略。在这里，我们提供的证据表明 MexT 序列多样性是 PAO1 谱系完整性的预测因子，并定义了该蛋白的原型序列。
