Nkuimi Wandjou Joice Guileine, Mevi Serena, Sagratini Gianni, Vittori Sauro, Dall'Acqua Stefano, Caprioli Giovanni, Lupidi Giulio, Mombelli Giacomo, Arpini Sabrina, Allegrini Pietro, Les Francisco, López Víctor, Maggi Filippo
School of Pharmacy, University of Camerino, Via S. Agostino 1, 62032 Camerino, Italy.
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy.
Plants (Basel). 2019 Dec 19;9(1):9. doi: 10.3390/plants9010009.
This study was undertaken to evaluate the nutraceutical potential of the Mela Rosa dei Monti Sibillini (MR), an ancient apple variety of the Sibillini Mountains, central Italy. The chemical profile of the apple's polyphenolic-rich extract (MRE) obtained from first- and second-choice samples using the Amberlite XAD7HP resin was analyzed by High Performance Liquid Chromatography with Diode-Array and Mass spectrometry (HPLC-DAD-MS) and 21 phytochemicals were quali-quantitatively determined. For comparative purposes, the polyphenol-rich extract of Annurca (ANE), a southern Italian variety, was analyzed. The antioxidant capacity of MREs was evaluated by Folin-Ciocalteu, 1, 1-diphenyl-2-picrylhydrazyl (DPPH), and 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. The inhibitory capacity of MREs for the enzymes α-glucosidase, lipase, monoamine oxidase A, tyrosinase, and acetylcholinesterase was also determined. The MREs showed higher polyphenolic and triterpene profiles than the ANE. Their radical scavenging activity was higher than that of ANE and comparable to the reference trolox. The MRE from the second-choice apples displayed higher contents of the 21 phytochemicals investigated. Either MRE from second-choice or first-choice samples showed enzymatic inhibition with IC values higher than those of reference inhibitors but worthy of nutraceutical consideration. Taken together, these results show the potential of MRE as a source of bioactive compounds to be used for pharmaceutical, nutraceutical, and cosmeceutical applications has been confirmed.
本研究旨在评估意大利中部锡比林尼山脉的古老苹果品种梅拉·罗莎·德伊·蒙蒂·锡比林尼（MR）的营养保健潜力。使用Amberlite XAD7HP树脂从一级和二级精选样品中获得的苹果富含多酚的提取物（MRE）的化学特征，通过配备二极管阵列和质谱的高效液相色谱法（HPLC-DAD-MS）进行分析，并对21种植物化学物质进行了定性和定量测定。为了进行比较，分析了意大利南部品种安努尔卡（ANE）的富含多酚的提取物。通过福林-西奥尔特法、1,1-二苯基-2-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）测定法评估了MRE的抗氧化能力。还测定了MRE对α-葡萄糖苷酶、脂肪酶、单胺氧化酶A、酪氨酸酶和乙酰胆碱酯酶的抑制能力。MRE显示出比ANE更高的多酚和三萜类成分。它们的自由基清除活性高于ANE，与参考物质生育三烯酚相当。二级精选苹果的MRE显示出所研究的21种植物化学物质的含量更高。二级精选或一级精选样品的MRE均显示出酶抑制作用，但IC值高于参考抑制剂，不过值得在营养保健方面加以考虑。综上所述，这些结果证实了MRE作为生物活性化合物来源在制药、营养保健和化妆品应用中的潜力。
