Delbue Serena, Franciotta Diego, Giannella Sara, Dolci Maria, Signorini Lucia, Ticozzi Rosalia, D'Alessandro Sarah, Campisciano Giuseppina, Comar Manola, Ferrante Pasquale, Ciotti Marco
Department of Biomedical, Surgical and Dental Sciences, University of Milano, Via Pascal, 36, 20133 Milano, Italy.
Neuroimmunology Laboratory, IRCCS Mondino Foundation, Via Mondino 2, 27100 Pavia, Italy.
Microorganisms. 2019 Dec 20;8(1):16. doi: 10.3390/microorganisms8010016.
Central nervous system (CNS) infections by human polyomaviruses (HPyVs), with the exception of JC (JCPyV), have been poorly studied.
In total, 234 cerebrospinal fluid (CSF) samples were collected from patients affected with neurological disorders. DNA was isolated and subjected to quantitative real-time PCR (Q-PCR) for the detection of six HPyVs: JCPyV, BKPyV, Merkel cell PyV (MCPyV), HPyV6, HPyV7, and HPyV9. Where possible, the molecular characterization of the viral strains was carried out by nested PCR and automated sequencing.
JCPyV was detected in 3/234 (1.3%), BKPyV in 15/234 (6.4%), MCPyV in 22/234 (9.4%), and HPyV6 in 1/234 (0.4%) CSF samples. JCPyV was detected at the highest ( < 0.05) mean load (3.7 × 10 copies/mL), followed by BKPyV (1.9 × 10 copies/mL), MCPyV (1.9 × 10 copies/mL), and HPyV6 (3.3 × 10 copies/mL). The noncoding control regions (NCCRs) of the sequenced viral strains were rearranged.
HPyVs other than JCPyV were found in the CSF of patients affected with different neurological diseases, probably as bystanders, rather than etiological agents of the disease. However, the fact that they can be latent in the CNS should be considered, especially in immunosuppressed patients.
除JC病毒(JCPyV)外,人类多瘤病毒(HPyV)引起的中枢神经系统(CNS)感染研究较少。
共收集了234例患有神经系统疾病患者的脑脊液(CSF)样本。提取DNA并进行定量实时PCR(Q-PCR)以检测六种HPyV:JCPyV、BKPyV、默克尔细胞多瘤病毒(MCPyV)、HPyV6、HPyV7和HPyV9。在可能的情况下,通过巢式PCR和自动测序对病毒株进行分子特征分析。
在234份CSF样本中,3份(1.3%)检测到JCPyV,15份(6.4%)检测到BKPyV,22份(9.4%)检测到MCPyV,1份(0.4%)检测到HPyV6。JCPyV的平均病毒载量最高(<0.05)(3.7×10拷贝/mL),其次是BKPyV(1.9×10拷贝/mL)、MCPyV(1.9×10拷贝/mL)和HPyV6(3.3×10拷贝/mL)。测序病毒株的非编码控制区(NCCR)发生了重排。
在患有不同神经系统疾病患者的CSF中发现了除JCPyV之外的HPyV,它们可能是旁观者,而非该疾病的病原体。然而,应考虑到它们可能潜伏在中枢神经系统,尤其是在免疫抑制患者中。