Deshmukh Ashlesha, Brown Logan, Barbe Mary F, Braverman Alan S, Tiwari Ekta, Hobson Lucas, Shunmugam Sudha, Armitage Oliver, Hewage Emil, Ruggieri Michael R, Morizio James
Triangle Biosystems, International, Durham, NC, United States.
Duke University, Viral Vector Core, Department of Neurobiology, Durham, NC, United States.
J Neurosci Methods. 2020 Mar 1;333:108562. doi: 10.1016/j.jneumeth.2019.108562. Epub 2019 Dec 17.
Peripheral nerve interfacing has many applications ranging from investigation of neural signals to therapeutic intervention for varied diseases. This need has driven technological advancements in the field of electrode arrays and wireless systems for in-vivo electrophysiological experiments. Hence we present our fully implantable, programmable miniaturized wireless stimulation and recording devices.
The method consists of technological advancements enabling implantable wireless recording up to 128 channels with a sampling rate of 50Khz and stimulation up to ±4 mA from 15 independent channels. The novelty of the technique consists of induction charging cages which enables freely moving small animals to undergo continuous electrophysiological and behavioral studies without any impediments. The biocompatible hermetic packaging technology for implantable capsules ensures stability for long-term chronic studies.
Electromyographs wirelessly recorded from leg muscles of a macaque and a rat using implantable technology are presented during different behavioral task studies. The device's simultaneous stimulation and recording capabilities are reported when interfaced with the vagus and pelvic nerves.
COMPARISON WITH EXISTING METHOD(S): The wireless interfacing technology has a large number of recording and stimulating channels without compromising on the signal quality due to sampling rates or stimulating current output capabilities. The induction charging technology along with transceiver and software interface allows experiments on multiple animals to be carried out simultaneously.
This customizable technology using wireless power transmission, reduced battery size, and miniaturized electronics has paved way for a robust, fully implantable, hermetic neural interface system enabling the study of bioelectronic medical therapies.
外周神经接口有许多应用，从神经信号研究到多种疾病的治疗干预。这种需求推动了用于体内电生理实验的电极阵列和无线系统领域的技术进步。因此，我们展示了我们完全可植入、可编程的小型化无线刺激和记录设备。
该方法包括技术进步，能够实现高达128通道的可植入无线记录，采样率为50Khz，以及来自15个独立通道的高达±4 mA的刺激。该技术的新颖之处在于感应充电笼，它能使自由活动的小动物不受任何阻碍地进行连续的电生理和行为研究。用于可植入胶囊的生物相容性密封包装技术确保了长期慢性研究的稳定性。
展示了在不同行为任务研究期间，使用可植入技术从猕猴和大鼠腿部肌肉无线记录的肌电图。报告了该设备与迷走神经和盆腔神经连接时的同时刺激和记录能力。
无线接口技术具有大量的记录和刺激通道，不会因采样率或刺激电流输出能力而影响信号质量。感应充电技术与收发器和软件接口一起，允许同时对多只动物进行实验。
这种使用无线电力传输、减小电池尺寸和小型化电子设备的可定制技术，为强大的、完全可植入的、密封的神经接口系统铺平了道路，从而能够开展生物电子医学疗法的研究。
