Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133, Milan, Italy.
Sci Rep. 2019 Dec 20;9(1):19525. doi: 10.1038/s41598-019-55975-1.
Plant polyphenolic compounds are considered a promising source for new antibacterial agents. In this study, we evaluated the antimicrobial activity of a collection of resveratrol-derived monomers and dimers screened as single molecules against a panel of nine foodborne pathogens. The results demonstrated that two monomers (i.e., pterostilbene 2 and (E)-3-hydroxy-4',5-dimethoxystilbene 9) and three dimers (i.e., δ-viniferin 10, viniferifuran 14 and dehydro-δ-viniferin 15) were endowed with significant antibacterial activity against gram-positive bacteria. The exposure of gram-positive foodborne pathogens to 100 µg/mL of 2, 9 and 15 induced severe cell membrane damage, resulting in the disruption of the phospholipid bilayer. The most promising dimeric compound, dehydro-δ-viniferin 15, was tested against Listeria monocytogenes, resulting in a loss of cultivability, viability and cell membrane potential. TEM analysis revealed grave morphological modifications on the cell membrane and leakage of intracellular content, confirming that the cell membrane was the principal biological target of the tested derivative.
植物多酚化合物被认为是新型抗菌剂的有前途的来源。在这项研究中，我们评估了一组白藜芦醇衍生的单体和二聚体作为单一分子对 9 种食源性病原体的抗菌活性。结果表明，两种单体（即紫檀芪 2 和（E）-3-羟基-4',5-二甲氧基二苯乙烯 9）和三种二聚体（即 δ-viniferin 10、viniferifuran 14 和去氢-δ-viniferin 15）对革兰氏阳性菌具有显著的抗菌活性。革兰氏阳性食源性病原体暴露于 100μg/mL 的 2、9 和 15 会导致严重的细胞膜损伤，破坏磷脂双层。最有前途的二聚体化合物去氢-δ-viniferin 15 被测试对李斯特菌的抑制作用，导致可培养性、生存力和细胞膜电位丧失。TEM 分析显示细胞膜严重的形态学改变和细胞内内容物的泄漏，证实细胞膜是测试衍生物的主要生物靶标。
