State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, No. 15 East Road of North Third Ring, Chao Yang District, Beijing 100029, China.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, No. 15 East Road of North Third Ring, Chao Yang District, Beijing 100029, China..
J Photochem Photobiol B. 2020 Jan;203:111743. doi: 10.1016/j.jphotobiol.2019.111743. Epub 2019 Dec 13.
The designing of new antibacterial agents with high and long-lasting activities are urgently needed in order to cope with the fast-emerging bacterial resistance. Zinc oxide nanoparticles (ZnO) have shown a significant promise as broad-spectrum antibacterial agents, and are efficient material in compromising bacterial membrane stability that leads to an increased cell permeability to nano-products. However, further engineering is required to improve their biological activities and to minimize their toxicity to healthy cells. In an attempt to resolve this issue, two semiconductor materials, ZnO and selenium (Se), were fabricated into a unique structural composite by a newly developed facile green method, and the designed composite was applied as an antibacterial nanomedicine. The developed methodology involves the initial preparation of ZnO, followed by its fabrication with Se at different temperatures (70 °C to 95 °C). Our experimental data showed that well defined interpenetrated crystalline Se network on ZnO (ZnO-Se) can be obtained at 80 °C for 180 min. The as-prepared ZnO-Se showed promising results in inhibiting the challenged bacterial strains under light irradiation (visible light) as compared to free ZnO. The enhanced biocidal property of ZnO-Se could be ascribed to its improved light-harvesting ability for sustainable induction of reactive oxygen species (ROS) and an active contact killing mechanism. Thus, ZnO-Se composite with a novel architecture could be a promising material in the treatment of bacterial infections by a mutual antibacterial synergy from the incorporated elements. Interestingly, the ZnO-Se has the ability to scavenge the overproduction of hydroxyl radicals, thus protecting the healthy cells from oxidative damage.
为了应对快速出现的细菌耐药性，急需设计具有高效持久活性的新型抗菌剂。氧化锌纳米粒子（ZnO）作为一种广谱抗菌剂显示出了巨大的应用潜力，它是一种有效的材料，可以破坏细菌膜的稳定性，导致纳米产物更容易进入细胞。然而，为了提高其生物活性并最大程度地降低对健康细胞的毒性，还需要进一步对其进行工程改造。为了解决这个问题，我们采用一种新开发的简便绿色方法，将两种半导体材料 ZnO 和硒（Se）制成独特的结构复合材料，并将设计的复合材料用作抗菌纳米药物。所开发的方法包括首先制备 ZnO，然后在不同温度（70°C 至 95°C）下用 Se 对其进行制造。我们的实验数据表明，在 80°C 下反应 180 分钟可以得到 ZnO 上具有良好定义的互穿结晶硒网络（ZnO-Se）。与游离 ZnO 相比，所制备的 ZnO-Se 在光照（可见光）下抑制受挑战的细菌菌株方面表现出了良好的效果。ZnO-Se 的杀菌性能增强可以归因于其提高了光捕获能力，从而可持续地诱导活性氧物种（ROS）并采用活性接触杀菌机制。因此，具有新型结构的 ZnO-Se 复合材料可以作为一种有前途的材料，通过掺入的元素的协同抗菌作用来治疗细菌感染。有趣的是，ZnO-Se 具有清除过多羟基自由基的能力，从而保护健康细胞免受氧化损伤。
