Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via E. Orabona 4, 70125, Bari, Italy.
The Faculty of Science and Engineering, Åbo Akademi University, Porthaninkatu 3, FI-20500, Turku, Finland.
Anal Bioanal Chem. 2020 Feb;412(4):811-818. doi: 10.1007/s00216-019-02319-7. Epub 2019 Dec 21.
Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance. Herein, a label-free single molecule detection of HIV-1 p24 capsid protein with a large (wide-field) single-molecule transistor (SiMoT) sensor is proposed. The system is based on an electrolyte-gated field-effect transistor whose gate is bio-functionalized with the antibody against the HIV-1 p24 capsid protein. The device exhibits a limit of detection of a single protein and a limit of quantification in the 10 molecule range. This study paves the way for a low-cost technology that can quantify, with single-molecule precision, the transition of a biological organism from being "healthy" to being "diseased" by tracking a target biomarker. This can open to the possibility of performing the earliest possible diagnosis.
早期诊断人类免疫缺陷病毒 1 型（HIV-1）感染对于实现高效治疗和限制病毒血症处于最高水平时疾病传播至关重要。为此，使用无标记、低成本和超灵敏筛选技术进行即时护理 HIV-1 检测将具有重要意义。在此，提出了一种使用大（宽场）单分子晶体管（SiMoT）传感器的无标记 HIV-1 p24 衣壳蛋白单分子检测方法。该系统基于栅极通过电解质门控的场效应晶体管，其栅极通过针对 HIV-1 p24 衣壳蛋白的抗体进行生物功能化。该器件的检测限为单个蛋白，定量限在 10 分子范围内。这项研究为一种低成本技术铺平了道路，该技术可以通过跟踪目标生物标志物，以单分子精度量化生物个体从“健康”到“患病”的转变，从而实现最早的诊断。
