Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), C/ del Til·lers, Campus UAB, 08193, Bellaterra, Barcelona, Spain.
Department of Chemistry, Università degli Studi di Bari A. Moro 4, Via Orabona, 70126, Bari, Italy; The Faculty of Science and Engineering, Åbo Akademi University, 20500, Turku, Finland.
Biosens Bioelectron. 2020 May 15;156:112103. doi: 10.1016/j.bios.2020.112103. Epub 2020 Feb 19.
A point-of-care (POC) device to enable de-centralized diagnostics can effectively reduce the time to treatment, especially in case of infectious diseases. However, many of the POC solutions presented so far do not comply with the ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment free, and deliverable to users) guidelines that are needed to ensure their on-field deployment. Herein, we present the proof of concept of a self-powered platform that operates using the analysed fluid, mimicking a blood sample, for early stage detection of HIV-1 infection. The platform contains a smart interfacing circuit to operate an ultra-sensitive electrolyte-gated field-effect transistor (EGOFET) as a sensor and facilitates an easy and affordable readout mechanism. The sensor transduces the bio-recognition event taking place at the gate electrode functionalized with the antibody against the HIV-1 p24 capsid protein, while it is powered via paper-based biofuel cell (BFC) that extracts the energy from the analysed sample itself. The self-powered platform is demonstrated to achieve detection of HIV-1 p24 antigens in fM range, suitable for early diagnosis. From these developments, a cost-effective digital POC device able to detect the transition from "healthy" to "infected" state at single-molecule precision, with no dependency on external power sources while using minimal components and simpler approach, is foreseen.
一种用于实现去中心化诊断的即时检测（POC）设备可以有效地缩短治疗时间，尤其是在传染病的情况下。然而，迄今为止提出的许多 POC 解决方案并不符合 ASSURED（负担得起、敏感、特异、用户友好、快速和稳健、无需设备且可交付给用户）准则，这些准则是确保其现场部署所必需的。在此，我们提出了一个自供电平台的概念验证，该平台使用所分析的流体（模拟血样）进行操作，用于早期 HIV-1 感染的检测。该平台包含一个智能接口电路，用于操作超灵敏的电解质门控场效应晶体管（EGOFET）作为传感器，并方便易于实现和负担得起的读出机制。传感器通过与 HIV-1 p24 衣壳蛋白抗体功能化的栅电极转换发生的生物识别事件，同时通过从分析样本本身提取能量的基于纸张的生物燃料电池（BFC）为其供电。该自供电平台可实现 fM 范围内 HIV-1 p24 抗原的检测，适用于早期诊断。从这些发展中，可以预见一种具有成本效益的数字 POC 设备，能够以单分子精度检测从“健康”到“感染”状态的转变，无需依赖外部电源，同时使用最少的组件和更简单的方法。
