Harima Hayato, Sasaki Michihito, Kajihara Masahiro, Mori-Kajihara Akina, Hang'ombe Bernard M, Changula Katendi, Orba Yasuko, Ogawa Hirohito, Simuunza Martin, Yoshida Reiko, Mweene Aaron, Takada Ayato, Sawa Hirofumi
Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, Zambia.
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan.
J Vet Med Sci. 2020 Feb 4;82(2):162-167. doi: 10.1292/jvms.19-0424. Epub 2019 Dec 20.
Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Phylogenetic analyses revealed that each of the identified orthoreoviruses formed a distinct branch among members of the Orthoreovirus genus. This is the first report that shrews are susceptible to orthoreovirus infection. Our results suggest the existence of undiscovered orthoreoviruses in shrews and provide important information about the genetic diversity of orthoreoviruses.
正呼肠孤病毒已在多种哺乳动物中被鉴定出来，然而，关于鼩鼱中正呼肠孤病毒的信息却尚无报道。在本研究中，我们对赞比亚的野生动物进行了筛查，包括鼩鼱、啮齿动物和蝙蝠，以检测正呼肠孤病毒。通过针对正呼肠孤病毒的RNA依赖性RNA聚合酶基因进行逆转录（RT）-PCR，从一份鼩鼱肠道内容物样本（24份中的1份）和一份蝙蝠结肠样本（96份中的1份）中检测到了两个正呼肠孤病毒RNA基因组。系统发育分析表明，每种鉴定出的正呼肠孤病毒在正呼肠孤病毒属成员中形成了一个独特的分支。这是关于鼩鼱易受正呼肠孤病毒感染的首次报道。我们的结果表明鼩鼱中存在未被发现的正呼肠孤病毒，并提供了有关正呼肠孤病毒遗传多样性的重要信息。
