Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
BMC Microbiol. 2018 Oct 17;18(1):135. doi: 10.1186/s12866-018-1302-9.
The pteropine orthoreovirus (PRV) was isolated from monkey (Macaca fascicularis) faecal samples collected from human-inhabited areas in Lopburi Province, Thailand. These samples were initially obtained to survey for the presence of hepatitis E virus (HEV).
Two virus isolates were retrieved by virus culture of 55 monkey faecal samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was successfully used to identify the viruses as the segmented dsRNA orthoreovirus. Phylogenetic analysis of the Lopburi orthoreovirus whole-genomes revealed relationships with the well-characterised PRVs Pulau (segment L1), Cangyuan (segments L2, M3 and S3), Melaka (segments L3 and M2), Kampar (segments M1 and S2) and Sikamat (segments S1 and S4) of Southeast Asia and China with nucleotide sequence identities of 93.5-98.9%. RT-PCR showed that PRV was detected in 10.9% (6/55) and HEV was detected in 25.5% (14/55) of the monkey faecal samples.
PRV was isolated from monkey faeces for the first time in Thailand via viral culture and LC-MS/MS. The genetic diversity of the virus genome segments suggested a re-assortment within the PRV species group. The overall findings emphasise that monkey faeces can be sources of zoonotic viruses, including PRV and HEV, and suggest the need for active virus surveillance in areas of human and monkey co-habitation to prevent and control emerging zoonotic diseases in the future.
从泰国洛布里省有人居住地区采集的猴（猕猴）粪便样本中分离出了翼手目正呼肠孤病毒（PRV）。这些样本最初是为了调查是否存在戊型肝炎病毒（HEV）而采集的。
从 55 份猴粪便样本的病毒培养中，分离出了两种病毒株。液相色谱-串联质谱（LC-MS/MS）成功地将这些病毒鉴定为有节段的 dsRNA 正呼肠孤病毒。对洛布里正呼肠孤病毒全基因组的系统进化分析显示，与已充分研究的东南亚和中国的普劳（L1 节段）、苍源（L2、M3 和 S3 节段）、马六甲（L3 和 M2 节段）、金宝（M1 和 S2 节段）和锡卡玛（S1 和 S4 节段）PRV 关系密切，核苷酸序列同一性为 93.5-98.9%。逆转录聚合酶链反应（RT-PCR）显示，在 10.9%（6/55）的猴粪便样本中检测到 PRV，在 25.5%（14/55）的样本中检测到 HEV。
通过病毒培养和 LC-MS/MS，首次在泰国从猴粪便中分离出 PRV。病毒基因组片段的遗传多样性表明，在 PRV 种内发生了重配。总体研究结果强调，猴粪便可能是包括 PRV 和 HEV 在内的人畜共患病病毒的来源，并表明需要在人与猴共存的地区积极进行病毒监测，以防止和控制未来新发的人畜共患疾病。
