Luo Ju-Yuan, Chen Gong-Sen, Liu Dong-Han, Mo Yu-Jia, Yu Xiang-Long, DU Shou-Ying, Bai Jie, Lu Yang, Wang Yu-Ming, Liu Cun
School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
Yifan Pharmaceutical Co.,Ltd. Lin'an 311300,China.
Zhongguo Zhong Yao Za Zhi. 2019 Sep;44(18):3994-3999. doi: 10.19540/j.cnki.cjcmm.20190522.302.
In recent years,the development and application of classical famous prescriptions have attracted much attention. However,the differences between ancient and modern conditions lead to difficulties in carrying out practical work. In this paper,with Houpu Wenzhong Decoction as an example,the key technologies of boiling granularity,water addition,boiling time and sample pretreatment methods were investigated on the basis of sufficient literature research. The experimental results showed that there was no significant difference in the concentration of index components between those with different granularity( 2 mm and 3-5 mm) and different decocting time( 30 min and 60 min),but the extraction rate of index components was relatively high when the granularity of powder was 2 mm and decocting time was 30 min. With the increase of water content,the concentration of index components and the extraction rate were increased in varying degrees. A certain proportion of methanol aqueous solution was used as the resolvent before content determination of the reference sample of Houpu Wenzhong Decoction,which could take into account both the spectral information of water-soluble components and fat-soluble components in the prescription,and help to display the overall information of the prescription' s chemical components more comprehensively. At the same time,the boiling and dispersing classical prescriptions in the Catalogue of Ancient Classical Prescriptions( the first batch) were collected and summarized in this study; the key influencing factors of decocting process were analyzed from different angles,and preliminary research suggestions were put forward,so as to provide a certain direction and reference for the establishment of quality standard of Houpu Wenzhong Decoction,as well as for the development,research and clinical application of boiling and dispersing classical prescriptions.
近年来，经典名方的开发与应用备受关注。然而，古今条件差异导致实际工作开展存在困难。本文以厚朴温中汤为例，在充分文献研究的基础上，对煮散粒度、加水量、煎煮时间及样品预处理方法等关键技术进行了考察。实验结果表明，不同粒度（2mm和3 - 5mm）和不同煎煮时间（30min和60min）的样品中指标成分浓度无显著差异，但粉末粒度为2mm且煎煮时间为30min时指标成分提取率相对较高。随着含水量增加，指标成分浓度和提取率均有不同程度提高。在厚朴温中汤对照品含量测定前，采用一定比例的甲醇水溶液作为溶剂，既能兼顾方中水溶性成分和脂溶性成分的光谱信息，又有助于更全面地展现方中化学成分的整体信息。同时，本研究对《古代经典名方目录》（第一批）中煮散类经典名方进行了收集汇总；从不同角度分析了煎煮过程中的关键影响因素，并提出了初步研究建议，以期为厚朴温中汤质量标准的建立以及煮散类经典名方的开发、研究和临床应用提供一定的方向和参考。
