Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai, PR China.
Cancer Invest. 2020 Jan;38(1):52-60. doi: 10.1080/07357907.2019.1709482. Epub 2020 Jan 20.
UHRF1 promotes melanoma progression by inducing cell proliferation, and is correlated with poor prognosis of melanoma patients. However, the regulation mechanism has not been fully elaborated. Here, we detected hsa-let-7b expression and its role in melanoma. Through Targetscan and miRanda predication, 30 overlapped miRNAs were found; further survival analysis revealed that hsa-let-7b was the only miRNA that affected the overall survival. Overexpressed hsa-let-7b could significantly inhibit the proliferation ability of A375 and A2058 cells, and this phenomenon was reversed after co-transfection with pLenti-UHRF1. In conclusion, hsa-let-7b regulates melanoma cells proliferation by targeting UHRF1.
UHRF1 通过诱导细胞增殖促进黑色素瘤的进展，并与黑色素瘤患者的不良预后相关。然而，其调控机制尚未充分阐述。在这里，我们检测了 hsa-let-7b 的表达及其在黑色素瘤中的作用。通过 Targetscan 和 miRanda 预测，发现了 30 个重叠的 miRNA；进一步的生存分析表明，hsa-let-7b 是唯一影响总生存期的 miRNA。过表达 hsa-let-7b 可显著抑制 A375 和 A2058 细胞的增殖能力，而与 pLenti-UHRF1 共转染后这种现象被逆转。综上所述，hsa-let-7b 通过靶向 UHRF1 调节黑色素瘤细胞的增殖。
