经球囊试验阻断后血浆中蛋白质变化的蛋白质组学分析。

Proteomic analysis of protein changes in plasma by balloon test occlusion.

机构信息

Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, Osaka, Japan; Department of Neurosurgery, Graduate School of Medicine, Osaka University, Japan; Department of Neurosurgery, Osaka International Cancer Institute, Osaka, Japan.

Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, Osaka, Japan; WPI Immunology Frontier Research Center, Osaka University, Japan; Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Neurology and Psychiatry, Kodaira, Japan.

出版信息

J Clin Neurosci. 2020 Feb;72:397-401. doi: 10.1016/j.jocn.2019.12.005. Epub 2019 Dec 23.

DOI:10.1016/j.jocn.2019.12.005

PMID:31875829

Abstract

Transient ischemia provides the tolerance against prolonged ischemia in the brain. In mouse experimental model, transient ischemia changes the composition ratio of circulating proteins, which associate with neuroprotection; however, the human evidence is lacking. Here we mimicked balloon test occlusion (BTO) of carotid artery as a transient ischemia and investigated the change of composition ratio of the circulating protein in the human plasma. We collected blood samples from nine patients (5 men and 4 women; mean age 64.2 years; range 45 to 77 years) before and 48 h after BTO and investigated the changes of circulating molecules level in the proteome using LC-MS/MS analysis. Leucine-rich alpha-2-glycoprotein and serum amyloid A-1 increased and protein AMBP decreased in the blood samples after BTO. Transient change of blood flow in the brain alters molecular expression in the plasma. Because the alteration of plasma protein composition is involved in ischemic tolerance in animal models, the proteins whose level was changed by BTO may be also involved in neuroprotection against ischemia in human.

摘要

短暂性脑缺血为大脑提供了对长时间脑缺血的耐受。在小鼠实验模型中，短暂性脑缺血改变了循环蛋白的组成比例，与神经保护有关；然而，人类的证据还缺乏。在这里，我们模拟了颈动脉球囊试验性闭塞（BTO）作为短暂性脑缺血，并研究了人类血浆中循环蛋白组成比例的变化。我们在 BTO 前和后 48 小时从 9 名患者（5 名男性和 4 名女性；平均年龄 64.2 岁；范围 45 至 77 岁）采集了血液样本，并使用 LC-MS/MS 分析研究了蛋白质组中循环分子水平的变化。BTO 后血液样本中亮氨酸丰富的α-2-糖蛋白和血清淀粉样蛋白 A-1 增加，AMBP 蛋白减少。脑血流的短暂变化改变了血浆中的分子表达。因为在动物模型中，血液蛋白组成的改变与缺血耐受有关，所以 BTO 改变水平的蛋白质也可能参与人类对缺血的神经保护。