脑缺血患者血浆蛋白质成分分析
Analysis of Blood Plasma Protein Composition in Patients with Cerebral Ischemia.
作者信息
Kisrieva Yu S, Petushkova N A, Samenkova N F, Kuznetsova G P, Larina O V, Teryaeva N B, Zgoda V G, Karuzina I I, Usachev D U, Belyaev A Yu
机构信息
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
N. N. Burdenko National Research and Practical Centre for Neurosurgery, Ministry of Health of the Russian Federation, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2018 May;165(1):22-26. doi: 10.1007/s10517-018-4090-1. Epub 2018 May 24.
Blood plasma proteome in patients with cerebral ischemia and healthy individuals was studied using comparative proteomic analysis based on tandem HPLC-MS/MS. Mass spectra were analysed in an automated mode using Progenesis LS-MS software and 256 proteins were identified. Significant quantitative differences were revealed for 20 proteins. It was found that changes in the blood plasma proteome in subjects with cerebral ischemia involved a wide range of proteins: molecular chaperones, fibrinolysis, angiogenesis, and immune system proteins, proteins involved in homeostasis maintenance, cell differentiation and proliferation, regulators of apoptosis, and cytoskeleton proteins.
采用基于串联HPLC-MS/MS的比较蛋白质组学分析方法，对脑缺血患者和健康个体的血浆蛋白质组进行了研究。使用Progenesis LS-MS软件以自动模式分析质谱，共鉴定出256种蛋白质。发现20种蛋白质存在显著的定量差异。结果表明，脑缺血患者血浆蛋白质组的变化涉及多种蛋白质：分子伴侣、纤维蛋白溶解、血管生成和免疫系统蛋白质、参与内环境稳态维持、细胞分化和增殖的蛋白质、细胞凋亡调节因子以及细胞骨架蛋白质。
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