Kisrieva Y S, Petushkova N A, Samenkova N F, Kuznetsova G P, Larina O V, Zavialova M G, Teryaeva N B, Belyaev A Y, Karuzina I I
Institute of Biomedical Chemistry, Moscow, Russia.
Burdenko Institute of Neurosurgery, Moscow, Russia.
Biomed Khim. 2016 Jul;62(5):599-602. doi: 10.18097/PBMC20166205599.
In the present study, we explored the technology of liquid chromatography-mass spectrometry (HPLC-MS/MS) for the proteome analysis of blood plasma of patients with early chronic cerebral ischemia. Analysis of mass-spectrometer data carried out in automatic mode using the software Progenesis LS-MS. As a result of this study identified 43 proteins. The differences identified in the study group compared with the control in 7 proteins. It was found that in the early stages of chronic cerebral ischemia proteome changes in blood plasma affect proteins related to the immune system, the system for the maintenance of hemostasis and lipid metabolism.
在本研究中，我们探索了液相色谱 - 质谱联用技术（HPLC-MS/MS）用于早期慢性脑缺血患者血浆蛋白质组分析。使用Progenesis LS-MS软件以自动模式对质谱仪数据进行分析。本研究结果鉴定出43种蛋白质。研究组与对照组相比，在7种蛋白质中发现了差异。研究发现，在慢性脑缺血的早期阶段，血浆蛋白质组变化影响与免疫系统、止血系统和脂质代谢相关的蛋白质。
