Negi Monika, Sepolia Namita, Panwar Sheilesh Singh, Kumar Mamit, Singla Jaya, Aggarwal Rajeev Kumar
Department of Oral Pathology, Microbiology and Forensic Odontology, HIDS, Paonta Sahib, Himachal Pradesh, India.
Bhojia Dental College and Hospital, Baddi, Himachal Pradesh, India.
J Family Med Prim Care. 2019 Dec 10;8(12):3956-3961. doi: 10.4103/jfmpc.jfmpc_667_19. eCollection 2019 Dec.
To evaluate and correlate the prevalence of species, salivary flow rate, pH, and buffer capacity of oral cavity in subjects with leukoplakia, tobacco pouch keratosis, and healthy controls.
In this prospective study, a total of 25 cases of leukoplakia, 25 of tobacco pouch keratosis, and 50 of healthy controls were studied. Sabouraud's dextrose agar (SDA) for the identification of and HiCrome differential Agar for the species identification of were used. Calculation of salivary flow rate, pH, and buffer capacity was done with Saliva-Check Buffer kit (GC).
The prevalence of carriage obtained was considerably greater in the study group associated with smokeless tobacco use. Non species predominated over in speciation among study groups on HiCrome agar and predominated among control groups. Statistically significant values were obtained by comparing the salivary flow rate, pH, and buffer capacity between the study groups and control groups.
The present study indicated prevalence was seen more in study group than healthy control groups, suggesting increased in smokeless tobacco users. The prevalence of Non species among leukoplakia and tobacco pouch keratosis indicates that there is pervasiveness of significant Non species in relation to salivary flow rate and buffer capacity. Therefore, the choice of antifungal treatment promptly must deal with not only resistance but, similarly, with the transformation in predominance from to Non species. The numbers of sample study were considerably less to conclude such result hence, numbers of studies should be performed at larger scale in the particular area.
评估并关联白斑、烟袋角化病患者及健康对照者口腔中念珠菌属的患病率、唾液流速、pH值和缓冲能力。
在这项前瞻性研究中，共研究了25例白斑患者、25例烟袋角化病患者和50例健康对照者。使用沙氏葡萄糖琼脂（SDA）鉴定念珠菌，使用HiCrome鉴别琼脂鉴定念珠菌属。使用唾液检测缓冲试剂盒（GC）计算唾液流速、pH值和缓冲能力。
在与无烟烟草使用相关的研究组中，念珠菌携带的患病率相当高。在HiCrome琼脂上的菌种鉴定中，非白色念珠菌在研究组中占主导地位，而在对照组中白色念珠菌占主导地位。通过比较研究组和对照组之间的唾液流速、pH值和缓冲能力，获得了具有统计学意义的值。
本研究表明，研究组中的念珠菌患病率高于健康对照组，这表明无烟烟草使用者中的念珠菌感染增加。白斑和烟袋角化病中非白色念珠菌的患病率表明，与唾液流速和缓冲能力相关的非白色念珠菌普遍存在。因此，抗真菌治疗的选择不仅要迅速应对耐药性，还要应对从白色念珠菌到非白色念珠菌的优势转变。由于样本研究数量较少，无法得出这样的结果，因此，应该在特定区域进行更大规模的研究。
