Wiench Rafał, Skaba Dariusz, Matys Jacek, Grzech-Leśniak Kinga
Department of Periodontal Diseases and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland.
Laser Laboratory Dental Surgery Department, Medical University of Wroclaw, 50-425 Wroclaw, Poland.
Antibiotics (Basel). 2021 Mar 25;10(4):349. doi: 10.3390/antibiotics10040349.
The effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral yeast infections was examined many times in recent years. The authors of this review tried to address the question: "Should TBO (toluidine blue ortho)-mediated aPDT be considered a possible alternative treatment for oral candidiasis?". PubMed/Medline and the Cochrane Central Register of Controlled Trials (CEN-TRAL) databases were searched from 1997 up to the 27th of October 2020 using a combination of the following keywords: (Candida OR Candidiasis oral OR Candidosis oral OR denture stomatitis) AND (toluidine blue OR photodynamic therapy OR aPDT OR photodynamic antimicrobial chemotherapy OR PACT OR photodynamic inactivation OR PDI). Animal studies or in vitro studies involving () and/or nonalbicans stain, randomized clinical trials (RCT) involving patients with oral candidiasis or denture stomatitis published solely in English language were included. elimination method in animal, in vitro studies and RCT used was TBO-mediated aPDT. Exactly 393 studies were taken into consideration. Then, after analyzing titles and abstracts of said studies, 361 were excluded. Only 32 studies ended up being selected for in-depth screening, after which 21 of them were included in this study. All studies reported the antifungal effectiveness of aPDT with TBO against and non-. In studies conducted with planktonic cells, only one study showed eradication of . All others showed partial elimination and only one of them was not statistically significant. Experiments on yeast biofilms, in all cases, showed partial, statistically significant cell growth inhibition and weight reduction (a reduction in the number of cells-mainly hyphae) and the mass of extracellular polymeric substance (EPS). In vivo aPDT mediated by TBO exhibits antifungal effects against oral spp.; however, its clinical effectiveness as a potent therapeutic strategy for oral yeast infections requires further investigation.
近年来，抗菌光动力疗法（aPDT）治疗口腔酵母菌感染的有效性已被多次研究。本综述的作者试图回答这个问题：“甲苯胺蓝邻苯二酚（TBO）介导的aPDT是否应被视为口腔念珠菌病的一种可能的替代治疗方法？” 使用以下关键词组合，检索了1997年至2020年10月27日的PubMed/Medline和Cochrane对照试验中央注册库（CENTRAL）数据库：（念珠菌或口腔念珠菌病或口腔念珠菌感染或义齿性口炎）和（甲苯胺蓝或光动力疗法或aPDT或光动力抗菌化疗或PACT或光动力灭活或PDI）。纳入涉及白色念珠菌和/或非白色念珠菌菌株的动物研究或体外研究，以及仅以英文发表的涉及口腔念珠菌病或义齿性口炎患者的随机临床试验（RCT）。动物、体外研究和RCT中使用的消除方法是TBO介导的aPDT。共考虑了393项研究。然后，在分析这些研究的标题和摘要后，排除了361项。最终仅32项研究被选入进行深入筛选，其中21项被纳入本研究。所有研究均报告了TBO介导的aPDT对白色念珠菌和非白色念珠菌的抗真菌有效性。在浮游细胞进行的研究中，只有一项研究显示白色念珠菌被根除。所有其他研究均显示部分消除，其中只有一项无统计学意义。在酵母生物膜上进行的实验在所有情况下均显示出部分、具有统计学意义的细胞生长抑制和重量减轻（细胞数量减少——主要是菌丝）以及细胞外聚合物（EPS）质量的减少。TBO介导的体内aPDT对口腔念珠菌属菌种具有抗真菌作用；然而，其作为口腔酵母菌感染有效治疗策略的临床有效性需要进一步研究。
