Wei Qiyao, Xu Chenchen, Wang Meiyan, Ye Haifeng
Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, China.
Sheng Wu Gong Cheng Xue Bao. 2019 Dec 25;35(12):2238-2256. doi: 10.13345/j.cjb.190298.
Dynamic variations of the cell microenvironment can affect cell differentiation, cell signaling pathways, individual growth, and disease. Optogenetics combines gene-encoded protein expression with optical controlling, and offers a novel, reversible, non-invasive and spatiotemporal-specific research tool to dynamically or reversibly regulate cell signaling pathways, subcellular localization and gene expression. This review summarizes the types of optogenetic components and the involved cellular signaling pathways, and explores the application and future prospects of the light-controlled cell signaling pathways.
细胞微环境的动态变化会影响细胞分化、细胞信号通路、个体生长和疾病。光遗传学将基因编码的蛋白质表达与光学控制相结合，提供了一种新颖、可逆、非侵入性且具有时空特异性的研究工具，用于动态或可逆地调节细胞信号通路、亚细胞定位和基因表达。本文综述了光遗传学组件的类型以及所涉及的细胞信号通路，并探讨了光控细胞信号通路的应用及未来前景。
