Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Granada, Spain.
Service of Medical Oncology, University Hospital Virgen de las Nieves of Granada, Granada, Spain.
Sci Rep. 2019 Dec 27;9(1):20198. doi: 10.1038/s41598-019-55952-8.
Colorectal cancer is one of the main causes of cancer death worldwide, and novel biomarkers are urgently needed for its early diagnosis and treatment. The utilization of metabolomics to identify and quantify metabolites in body fluids may allow the detection of changes in their concentrations that could serve as diagnostic markers for colorectal cancer and may also represent new therapeutic targets. Metabolomics generates a pathophysiological 'fingerprint' that is unique to each individual. The purpose of our study was to identify a differential metabolomic signature for metastatic colorectal cancer. Serum samples from 60 healthy controls and 65 patients with metastatic colorectal cancer were studied by liquid chromatography coupled to high-resolution mass spectrometry in an untargeted metabolomic approach. Multivariate analysis revealed a separation between patients with metastatic colorectal cancer and healthy controls, who significantly differed in serum concentrations of one endocannabinoid, two glycerophospholipids, and two sphingolipids. These findings demonstrate that metabolomics using liquid-chromatography coupled to high-resolution mass spectrometry offers a potent diagnostic tool for metastatic colorectal cancer.
结直肠癌是全球癌症死亡的主要原因之一，因此迫切需要新的生物标志物来进行早期诊断和治疗。利用代谢组学来鉴定和定量体液中的代谢物，可以检测到其浓度的变化，这些变化可以作为结直肠癌的诊断标志物，也可能代表新的治疗靶点。代谢组学生成的病理生理学“指纹”是每个人所特有的。本研究的目的是确定转移性结直肠癌的差异代谢组学特征。采用液相色谱-高分辨质谱联用的非靶向代谢组学方法，对 60 例健康对照者和 65 例转移性结直肠癌患者的血清样本进行了研究。多变量分析显示，转移性结直肠癌患者与健康对照者之间存在分离，转移性结直肠癌患者血清中一种内源性大麻素、两种甘油磷脂和两种神经鞘脂的浓度显著不同。这些发现表明，液相色谱-高分辨质谱联用的代谢组学为转移性结直肠癌提供了一种强有力的诊断工具。
