Center for Plant Cell Biology, Institute of Integrative Genome Biology, Department of Botany and Plant Sciences, University of California, Riverside, Riverside, California, United States of America.
College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou, Fujian, China.
Sci Rep. 2019 Dec 27;9(1):20083. doi: 10.1038/s41598-019-56401-2.
Dubbed as a "global destroyer of crops", the soil-borne fungus Macrophomina phaseolina (Mp) infects more than 500 plant species including many economically important cash crops. Host defenses against infection by this pathogen are poorly understood. We established interactions between Mp and Arabidopsis thaliana (Arabidopsis) as a model system to quantitatively assess host factors affecting the outcome of Mp infections. Using agar plate-based infection assays with different Arabidopsis genotypes, we found signaling mechanisms dependent on the plant hormones ethylene, jasmonic acid and salicylic acid to control host defense against this pathogen. By profiling host transcripts in Mp-infected roots of the wild-type Arabidopsis accession Col-0 and ein2/jar1, an ethylene/jasmonic acid-signaling deficient mutant that exhibits enhanced susceptibility to this pathogen, we identified hundreds of genes potentially contributing to a diverse array of defense responses, which seem coordinated by complex interplay between multiple hormonal response-pathways. Our results establish Mp/Arabidopsis interactions as a useful model pathosystem, allowing for application of the vast genomics-related resources of this versatile model plant to the systematic investigation of previously understudied host defenses against a major crop plant pathogen.
被称为“全球作物破坏者”的土传真菌旋孢腔菌（Mp）感染了包括许多重要经济作物在内的 500 多种植物。人们对宿主抵御这种病原体感染的机制知之甚少。我们建立了 Mp 与拟南芥（Arabidopsis）之间的相互作用，作为一个模型系统，以定量评估影响 Mp 感染结果的宿主因素。通过使用不同拟南芥基因型的基于琼脂平板的感染测定，我们发现依赖于植物激素乙烯、茉莉酸和水杨酸的信号机制控制宿主对这种病原体的防御。通过对野生型拟南芥品系 Col-0 和乙烯/茉莉酸信号缺陷突变体 ein2/jar1 中 Mp 感染根的宿主转录物进行分析，我们鉴定了数百个可能有助于多种防御反应的基因，这些反应似乎通过多种激素反应途径的复杂相互作用来协调。我们的研究结果确立了 Mp/拟南芥的相互作用作为一个有用的模式病理系统，允许将这种多功能模式植物的大量与基因组相关的资源应用于对以前研究较少的主要作物植物病原体的宿主防御的系统研究。
