Department of Health Care, Qingdao Municipal Hospital (East), Qingdao, Shandong, China.
Department of General surgery, The affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
Bioengineered. 2020 Dec;11(1):53-60. doi: 10.1080/21655979.2019.1705054.
Long non-coding RNAs (lncRNAs) recently have been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. Here we identified LOC285194 as a key regulator of cell proliferation and apoptosis during atherosclerosis. The expression of LOC285194 was dramatically down-regulated in a aortic atherosclerotic plaques of well-defined model of apolipoprotein-E knockout (ApoE) mice. Moreover, we found that targeting LOC285194 results in neointimal hyperplasia in carotid artery injury model. We also showed that targeting LOC285194 promotes cell proliferation and inhibits apoptosis in vascular smooth muscle cells (VSMCs) . In addition, targeting LOC285194 promotes cell invasion and migration in vitro. Our studies identify LOC285194 as a novel regulator of cell proliferation and apoptosis and suggest that this lncRNA could serve as a therapeutic target to treat atherosclerosis and related cardiovascular disorders.
长链非编码 RNA（lncRNA）最近被牵涉到许多生物过程和疾病中。动脉粥样硬化是心血管疾病的主要危险因素。然而，lncRNA 在动脉粥样硬化中的功能作用在很大程度上是未知的。在这里，我们确定 LOC285194 是动脉粥样硬化过程中细胞增殖和凋亡的关键调节因子。LOC285194 的表达在载脂蛋白 E 敲除（ApoE）小鼠明确的动脉粥样硬化模型的主动脉粥样硬化斑块中显著下调。此外，我们发现靶向 LOC285194 会导致颈动脉损伤模型中的新生内膜过度增生。我们还表明，靶向 LOC285194 可促进血管平滑肌细胞（VSMC）中的细胞增殖和抑制细胞凋亡。此外，靶向 LOC285194 可促进体外细胞侵袭和迁移。我们的研究确定 LOC285194 是细胞增殖和凋亡的新型调节因子，并表明该 lncRNA 可作为治疗动脉粥样硬化和相关心血管疾病的治疗靶点。
