体外消化和发酵从德氏乳杆菌保加利亚亚种 SRFM-1 中释放的胞外多糖（r-EPS）。
In vitro digestion and fermentation of released exopolysaccharides (r-EPS) from Lactobacillus delbrueckii ssp. bulgaricus SRFM-1.
机构信息
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
School of Food Science and Pharmacy, Xinjiang Agricultural University, Urumchi, Xinjiang 830052, PR China.
出版信息
Carbohydr Polym. 2020 Feb 15;230:115593. doi: 10.1016/j.carbpol.2019.115593. Epub 2019 Nov 11.
The aim of this study was to investigate the in vitro digestion and fermentation prebiotic properties of three released-exopolysaccharide fractions (r-EPS1, r-EPS2 and r-EPS3) from Lactobacillus delbrueckii ssp. bulgaricus SRFM-1. There were no free oligosaccharides and/or monosaccharides for r-EPS1 before and after simulated buccal, gastric and small intestinal (GSI) digestion in vitro. In contrast, r-EPS2 (13.4 %) and r-EPS3 (10.6 %) generated a few monosaccharides after digestion. Additionally, r-EPS1 and r-EPS2 seemed to present a strong bifidogenic effect comparing to inulin, as they exhibited high values of selectivity index (13.17 and 12.84, respectively). Furthermore, the fermentation with r-EPS1 produced the highest contents of acetic acid and lactic acid (56.3 mM and 44.29 mM, respectively), which resulted in the highest amounts of total short chain fatty acid (145.51 mM) followed by r-EPS2 (135.57 mM) and inulin (99.28 mM). These results indicated that r-EPS from L. delbrueckii ssp. bulgaricus SRFM-1 could be a good potential candidate for new functional food prebiotic.
本研究旨在探究从德氏乳杆菌保加利亚亚种 SRFM-1 中释放的三种胞外多糖（r-EPS1、r-EPS2 和 r-EPS3）的体外消化和发酵特性。r-EPS1 在模拟口腔、胃和小肠（GSI）消化前后均未检测到游离低聚糖和/或单糖。相比之下，r-EPS2（13.4%）和 r-EPS3（10.6%）在消化后产生了一些单糖。此外，与菊粉相比，r-EPS1 和 r-EPS2 似乎具有较强的双歧杆菌增殖作用，其选择性指数分别高达 13.17 和 12.84。此外，r-EPS1 的发酵产生了最高含量的乙酸和乳酸（分别为 56.3mM 和 44.29mM），导致总短链脂肪酸含量最高（145.51mM），其次是 r-EPS2（135.57mM）和菊粉（99.28mM）。这些结果表明，德氏乳杆菌保加利亚亚种 SRFM-1 的 r-EPS 可能是一种有潜力的新型功能性食品益生元候选物。
Zotero 插件