石墨烯/壳聚糖纳米复合材料对产生物膜的铜绿假单胞菌和肺炎克雷伯菌的抗生物膜研究。
Anti-biofilm investigation of graphene/chitosan nanocomposites against biofilm producing P. aeruginosa and K. pneumoniae.
机构信息
Laboratorio de Nanocelulosa y Biomateriales, Departamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Avenida Beauchef 851, Santiago, Chile.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China; Medical Microbiology & Marine Pharmacology Laboratory, Department of Marine Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, PR China.
出版信息
Carbohydr Polym. 2020 Feb 15;230:115646. doi: 10.1016/j.carbpol.2019.115646. Epub 2019 Nov 22.
In this study graphene/chitosan nanoparticles (GR/CS NCs) were developed. The homogenous combination of GR and CS was confirmed by FTIR spectroscopy. The combination of CS with GR sheets reduced the XRD intensity of the GR peak in GR/CS NCs, while TEM images revealed the immobile CS coating of GR sheets. Further, the anti-biofilm activity of GR/CS NCs was tested. The tests showed that the formation of biofilm by Pseudomonas aeruginosa and Klebsiella pneumoniae was inhibited at 40□g/mL GR/CS NCs up to 94 and 92 %, respectively. The intracellular and cell surface damage of the bacteria was observed by CLSM and SEM. Also, GR/CS NCs produced a toxic effect of 90 % on Artemia franciscana at 70□g/mL upon 24 h incubation. The recorded properties of the synthesized GR/CS NCs qualify them as potential agents against multi-drug resistant bacteria.
在这项研究中，制备了石墨烯/壳聚糖纳米粒子（GR/CS NCs）。傅里叶变换红外光谱（FTIR）证实了 GR 和 CS 的均匀结合。CS 与 GR 片的结合降低了 GR/CS NCs 中 GR 峰的 XRD 强度，而 TEM 图像显示 GR 片上的 CS 涂层不移动。此外，还测试了 GR/CS NCs 的抗生物膜活性。结果表明，浓度为 40□g/mL 的 GR/CS NCs 对铜绿假单胞菌和肺炎克雷伯菌生物膜的形成抑制率分别高达 94%和 92%。通过 CLSM 和 SEM 观察到细菌的细胞内和细胞表面损伤。此外，在 70□g/mL 浓度下孵育 24 小时后，GR/CS NCs 对卤虫的致死率为 90%。所合成的 GR/CS NCs 的这些特性使其有潜力成为对抗多药耐药菌的药物。
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