College of Food Science and Engineering, Jilin University, Changchun, Jilin, 130062, China.
Department of Nutrition and Food Science, College of Agriculture and Life Sciences, University of Vermont, Burlington, VT, 05405, USA; Key Laboratory of Dairy Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China.
Carbohydr Polym. 2020 Feb 15;230:115680. doi: 10.1016/j.carbpol.2019.115680. Epub 2019 Nov 27.
Three main fractions, one neutral polysaccharide (ACPN-1a) and two acidic polysaccharides (ACPA-1a and ACPA-2a), were isolated from crude polysaccharides of Auricularia cornea var. Li. (ACP). The physicochemical properties and structural characteristics of these fractions were investigated using High Performance Gel Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC), Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), and Nuclear Magnetic Resonance (NMR) spectra. The results showed that ACPN-1a with a higher molecular weight (∼2.18 × 10 Da) was a β-(1→3)-glucan with (1→6)-linked β-Glcp residues for side chains. ACPA-1a was a heteropolysaccharide and had the backbone of (1→3)-linked β-Glcp and (1→6)-linked α-Manp and side chains with β-Xylp or β-GlcpA residues. ACPA-2a had the average molecular weight of 8.5 × 10 Da and was composed of a (1→6)-linked α-Manp backbone and side chains with β-Xylp or β-GlcpA residues. The data provided useful information for further investigation on the structure-activity relationship of Auricularia cornea var. Li. polysaccharides.
从银耳（Auricularia cornea var. Li.）粗多糖中分离得到三种主要的多糖组分，一种中性多糖（ACPN-1a）和两种酸性多糖（ACPA-1a 和 ACPA-2a）。采用高效凝胶渗透色谱（HPGPC）、高效液相色谱（HPLC）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和核磁共振（NMR）光谱等方法对这些组分的理化性质和结构特征进行了研究。结果表明，分子量较高（约 2.18×10 Da）的 ACPN-1a 是一种β-（1→3）-葡聚糖，具有（1→6）连接的β-Glcp 残基作为侧链。ACPA-1a 是一种杂多糖，其主链为（1→3）连接的β-Glcp 和（1→6）连接的α-Manp，侧链为β-Xylp 或β-GlcpA 残基。ACPA-2a 的平均分子量为 8.5×10 Da，由（1→6）连接的α-Manp 主链和β-Xylp 或β-GlcpA 残基的侧链组成。这些数据为进一步研究银耳多糖的结构-活性关系提供了有用的信息。
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