Li Yutong, Li Shangkun, Li Di, Gao Yuan, Kong Shuhua, Liu Jingyi, Liu Shu, Ma Yichao, Zhou Hui, Ren Dandan, Wang Qiukuan, He Yunhai
College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China.
Foods. 2024 Sep 23;13(18):3019. doi: 10.3390/foods13183019.
In this study, a quantitative method based on fluorescein isothiocyanate (FITC)-labelled polyphenol-polysaccharide complex (HPC) and its purified fractions (PC1, PC4) was used, and its pharmacokinetics and tissue distribution were investigated in mice. The results showed that the FITC-labelled method had good linearity (R > 0.99), intra-day and inter-day precision (RSD, %) consistently lower than 15%, recovery (93.19-106.54%), and stability (RSD < 15%), which met the basic criteria for pharmacokinetic studies. The pharmacokinetic and tissue distribution results in mice after administration showed that all three sample groups could enter the blood circulation. and HPC-FITC had a longer half-life (T: 26.92 ± 0.76 h) and mean retention time (MRT: 36.48 h) due to its larger molecular weight. The three groups of samples could be absorbed by the organism in a short time (0.5 h) mainly in the stomach and intestine; the samples could be detected in the urine after 2 h of administration indicating strong renal uptake, and faecal excretion reached its maximum at 12 h. The samples were also detected in the urine after 2 h of administration. This study provides some theoretical basis for the tissue distribution pattern of polyphenol-polysaccharide complex.
在本研究中，使用了一种基于异硫氰酸荧光素（FITC）标记的多酚 - 多糖复合物（HPC）及其纯化组分（PC1、PC4）的定量方法，并在小鼠中研究了其药代动力学和组织分布。结果表明，FITC标记法具有良好的线性（R > 0.99），日内和日间精密度（RSD，%）始终低于15%，回收率（93.19 - 106.54%）和稳定性（RSD < 15%），符合药代动力学研究的基本标准。给药后小鼠的药代动力学和组织分布结果表明，所有三个样品组均可进入血液循环。并且由于HPC - FITC分子量较大，其半衰期较长（T：26.92 ± 0.76 h），平均滞留时间较长（MRT：36.48 h）。三组样品在短时间（0.5 h）内主要在胃和肠道被机体吸收；给药2 h后可在尿液中检测到样品，表明肾脏摄取较强，粪便排泄在12 h达到最大值。给药2 h后也可在尿液中检测到样品。本研究为多酚 - 多糖复合物的组织分布模式提供了一些理论依据。
