University of Maribor, Faculty of Medicine, Department of Pharmacology, Taborska ulica 8, 2000 Maribor, Slovenia; University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Taborska ulica 8, 2000 Maribor, Slovenia.
University of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Analytical Chemistry and Industrial Analysis, Smetanova ulica 17,2000 Maribor, Slovenia; University of Tirana, Faculty of Natural Sciences, Boulevard "Zogu I", 1001 Tirana, Albania.
Carbohydr Polym. 2020 Feb 15;230:115612. doi: 10.1016/j.carbpol.2019.115612. Epub 2019 Nov 13.
This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose. Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method. The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition. Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample. The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity. Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.
本工作报道了一种新型多层涂层的制备和系统测试，该涂层由非甾体抗炎药双氯芬酸和生物聚合物羧甲基纤维素组成。药物释放测试是在自动化透皮扩散细胞采样系统上进行的，结合紫外可见光谱法作为释放药物浓度的测定方法。结果表明，大部分药物在前 6 小时内释放，而通过改变多层涂层的组成可以调整总的释放量。对人成骨细胞进行的生物相容性测试表明，与对照样品相比，细胞活力提高了 7%至 17%。为了获得更多关于所制备材料促进骨整合能力的证据，还进行了在细胞外基质产生和功能中起重要作用的蛋白质表达实验。最后，电化学测量证实涂层不会影响 AISI 316LVM 不锈钢的腐蚀敏感性。
