Kızıldağ Alper, Arabacı Taner, Albayrak Mevlüt, Taşdemir Ufuk, Şenel Erman, Dalyanoglu Mukaddes, Demirci Elif
Department of Periodontology, Faculty of Dentistry, Pamukkale University, Denizli, Turkey.
Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
J Dent Sci. 2019 Dec;14(4):339-345. doi: 10.1016/j.jds.2019.03.011. Epub 2019 May 7.
BACKGROUND/PURPOSE: Caffeic acid phenethyl ester (CAPE) is an antioxidant which is decreases the bone resorption and enhances the bone healing. The aim of this study was to investigate the effects of administering systemic CAPE on alveolar bone loss in rats with experimental periodontitis.
Thirty male Sprague Dawley rats were divided into three groups: control, endotoxin-induced periodontitis (EP), and EP treated with CAPE (EP-CAPE). Endotoxin was injected into the gingiva of test rats on days 1, 3, and 5, whereas saline was injected into the control rats. The EP-CAPE group received 10 mmol/kg/day CAPE intraperitoneally for 28 consecutive days. Saline was given in the control and EP groups in the same manner. At the end of the study, intracardiac blood samples were obtained, and the rats were sacrificed. Alveolar bone loss was analyzed with histometric measurements. The oxidative stress index (OSI) was used to evaluate the oxidative stress. The receptor activator of the nuclear factor kappa B ligand (RANKL) level was analyzed stereologically.
CAPE administration significantly decreased the serum OSI and interleukin-1β levels. Alveolar bone loss was statistically higher in the EP group compared with the EP-CAPE group ( < 0.05). Immunohistochemical analyses of the RANKL were significantly lower in the EP-CAPE group than in the EP group ( < 0.05).
This experimental study revealed that CAPE administration significantly prevented alveolar bone loss and stimulated periodontal tissue healing.
背景/目的：咖啡酸苯乙酯（CAPE）是一种抗氧化剂，可减少骨吸收并促进骨愈合。本研究旨在探讨全身给予CAPE对实验性牙周炎大鼠牙槽骨丧失的影响。
30只雄性Sprague Dawley大鼠分为三组：对照组、内毒素诱导的牙周炎组（EP）和CAPE治疗的EP组（EP-CAPE）。在第1、3和5天向试验大鼠的牙龈注射内毒素，而向对照大鼠注射生理盐水。EP-CAPE组连续28天腹腔注射10 mmol/kg/天的CAPE。对照组和EP组以相同方式给予生理盐水。在研究结束时，采集心脏内血液样本，并处死大鼠。通过组织计量学测量分析牙槽骨丧失情况。使用氧化应激指数（OSI）评估氧化应激。立体分析核因子κB受体活化因子配体（RANKL）水平。
给予CAPE可显著降低血清OSI和白细胞介素-1β水平。与EP-CAPE组相比，EP组的牙槽骨丧失在统计学上更高（<0.05）。EP-CAPE组RANKL的免疫组织化学分析显著低于EP组（<0.05）。
本实验研究表明，给予CAPE可显著预防牙槽骨丧失并促进牙周组织愈合。
