Espinoza Friederike, Vidal Stefan, Rautenbach Fanie, Lewu Francis, Nchu Felix
Department of Crop Sciences, Section of Agricultural Entomology, Georg-August University, Grisebachstrasse 6, 37077, Goettingen, Germany.
Department of Biomedical Sciences, Cape Peninsula University of Technology, Symphony Way, Bellville, P.O. Box 1906, Bellville 7535, South Africa.
Heliyon. 2019 Dec 18;5(12):e03038. doi: 10.1016/j.heliyon.2019.e03038. eCollection 2019 Dec.
The endophytic plant-fungi symbiotic relationship can be explored to improve cultivation of targeted medicinal plant species. The objective of this study was to assess the effects of the cultivation of chive () in plant growth medium inoculated with the entomopathogenic fungus, (Hypocreales). Twelve replicates of chive seedlings were exposed to inoculum formulated at concentrations of 0, 1 × 10, 1 × 10 and 1 × 10 conidia mL in a completely randomized design. We assessed plant growth parameters, such as leaf number and plant height weekly and root length, leaf and root fresh and dry weights and secondary metabolites three weeks post-fungal inoculation. The fungus was re-isolated from some of the leaves and roots of the treated plants suggesting that the fungus successfully colonized the plant tissue. Generally, the results indicated that the fungal inoculation had minimal effect on most of the growth parameters assessed in relation to the control. Remarkably, plants exposed to the fungus recorded greater (p < 0.05) total alkaloid, ranging from 2.98 - 3.76 mg atropine equivalent (AE)/g dry weight (DW) compared to the control plants (1.96 mg AE/g DW) for the leaves. This study demonstrated that endophytic fungi could be used to improve the yield of active chemical constituents in cultivated medicinal plants.
可以探索内生植物与真菌的共生关系，以改善目标药用植物品种的种植。本研究的目的是评估在接种昆虫病原真菌球孢白僵菌（Hypocreales）的植物生长培养基中种植细香葱（）的效果。采用完全随机设计，将12份细香葱幼苗分别暴露于浓度为0、1×10、1×10和1×10分生孢子/mL的球孢白僵菌接种物中。在真菌接种三周后，我们每周评估植物生长参数，如叶片数量和株高，以及根长、叶和根的鲜重和干重，还有次生代谢产物。从处理过的植物的一些叶子和根中重新分离出了这种真菌，这表明该真菌成功地定殖于植物组织中。总体而言，结果表明，与对照相比，真菌接种对所评估的大多数生长参数影响极小。值得注意的是，与对照植物（叶片中1.96毫克阿托品当量（AE）/克干重）相比，接触该真菌的植物叶片中的总生物碱含量更高（p<0.05），范围为2.98 - 3.76毫克AE/克干重。本研究表明，内生真菌可用于提高栽培药用植物中活性化学成分的产量。
