Zhang Wenwen, Chen Yuanyuan, Shi Qing, Hou Benxiang, Yang Qiubo
Beijing Institute for Dental Research, Capital Medical University, School of Stomatology, Beijing, China.
Department of Pediatric Dentistry, Capital Medical University, School of Stomatology, Beijing, China.
Microb Pathog. 2020 Apr;141:103954. doi: 10.1016/j.micpath.2019.103954. Epub 2019 Dec 28.
This study aims to detect the predominant bacteria in acute periapical abscesses of primary teeth using culture-independent molecular methods based on 16S ribosomal DNA cloning.
Purulent material was collected from nine children diagnosed with abscesses of endodontic origin. DNA was extracted and the 16S rRNA gene amplified with universal primer pairs 27F and 1492R. Amplified genes were cloned, sequenced by Applied Biosystems, and identified by comparison with known 16S rRNA gene sequences.
Bacterial DNA was present in all nine purulence samples. A total of 681 clones were classified into 8 phyla, 78 genera, and 125 species/phylotypes. The phyla were Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, Actinobacteria, Tenericutes, Deinococcus-Thermus, and Spirochaetes. The most dominant genera were Streptococcus (13.3%), Fusobacterium (11.8%), Parvimonas (7.8%), Prevotella (6.7%), Sphingomonas (5.8%), and Hafnia (5.2%). Fusobacterium nucleatum (11.5%), Parvimonas micra (7.8%), Streptococcus intermedius (6.6%), Sphingomonas echinoides (5.3%), Hafnia alvei (5.2%), and Citrobacter freundii (4.9%) were the most common species/phylotypes. Among these species/phylotypes, F.nucleatum was the most prevalent (7/9). C. freundii, Carnobacterium maltaromaticum, and H. alvei were seldom detected species in acute periapical abscesses but had relatively high abundance and prevalence.
Acute periapical abscesses are polymicrobial with certain prevalent bacteria, especially anaerobic bacterium. The most predominant and prevalent bacteria of acute periapical abscesses in children was F. nucleatum.
本研究旨在使用基于16S核糖体DNA克隆的非培养分子方法，检测乳牙急性根尖周脓肿中的主要细菌。
从9名被诊断为牙髓源性脓肿的儿童中收集脓性物质。提取DNA，并用通用引物对27F和1492R扩增16S rRNA基因。扩增的基因被克隆，由应用生物系统公司进行测序，并通过与已知的16S rRNA基因序列进行比较来鉴定。
所有9个脓性样本中均存在细菌DNA。总共681个克隆被分类为8个门、78个属和125个种/系统型。这些门包括厚壁菌门、变形菌门、梭杆菌门、拟杆菌门、放线菌门、柔膜菌门、嗜热栖热菌门和螺旋体门。最主要的属是链球菌属（13.3%）、梭杆菌属（11.8%）、小单胞菌属（7.8%）、普雷沃菌属（6.7%）、鞘氨醇单胞菌属（5.8%）和哈夫尼亚菌属（5.2%）。具核梭杆菌（11.5%）、微小单胞菌（7.8%）、中间链球菌（6.6%）、棘鞘氨醇单胞菌（5.3%）、蜂房哈夫尼亚菌（5.2%）和弗氏柠檬酸杆菌（4.9%）是最常见的种/系统型。在这些种/系统型中，具核梭杆菌最为普遍（7/9）。弗氏柠檬酸杆菌、麦芽香肉杆菌和蜂房哈夫尼亚菌在急性根尖周脓肿中是很少检测到的菌种，但具有相对较高的丰度和流行率。
急性根尖周脓肿是由多种细菌引起的，有某些常见细菌，尤其是厌氧菌。儿童急性根尖周脓肿中最主要和最普遍的细菌是具核梭杆菌。
