牙周炎通过肠道微生物群和粪便代谢组与良性前列腺增生相关。

Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome.

作者信息

Guo Xing-Pei, Yang Jun, Wu Lan, Fang Cheng, Gu Jia-Min, Li Fei, Liu Han-Song, Li Lu-Yao, Wang Shuang-Ying

机构信息

Department of General Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, China.

Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.

出版信息

Front Microbiol. 2023 Dec 13;14:1280628. doi: 10.3389/fmicb.2023.1280628. eCollection 2023.

DOI:10.3389/fmicb.2023.1280628

PMID:38163068

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10756679/

Abstract

OBJECTIVES

Periodontitis is associated with benign prostatic hyperplasia (BPH), whether it related to gut floramicrobiota and metabonomics is unclear.

METHODS

We established ligature-induced periodontitis (EP), testosterone-induced BPH, and composite rat models. Fecal samples were collected to detect gut microbiota by 16S rDNA sequencing and metabonomics were detected by liquid chromatography tandem mass spectrometry (LC-MS/MS).

RESULTS

Sequencing results revealed differential gut floramicrobiota composition between EP+BPH group and other three groups. The abundances of were significantly increased in EP+BPH group compared with other groups. , and were significantly decreased in EP+BPH group compared with BPH group, while and Escherichia were significantly decreased compared with EP group. For gut metabonomics, LC-MS/MS showed that fecal metabolites and seven metabolic pathways were changed in EP+BPH group, such as biosynthesis of unsaturated fatty acids, steroid hormone biosynthesis. Correlation analysis showed that the alterations of gut metabolism were significantly correlated with differential gut floramicrobiota, such as and .

CONCLUSION

Our study highlights the relationship of periodontitis and BPH, the alterations of gut floramicrobiota and metabolites may be involved in two diseases, which provides new idea for prevention and treatment of patients with periodontitis concurrent BPH.

摘要

目的

牙周炎与良性前列腺增生（BPH）相关，但其是否与肠道菌群和代谢组学有关尚不清楚。

方法

我们建立了结扎诱导的牙周炎（EP）、睾酮诱导的BPH以及复合大鼠模型。收集粪便样本，通过16S rDNA测序检测肠道微生物群，并通过液相色谱串联质谱（LC-MS/MS）检测代谢组学。

结果

测序结果显示，EP+BPH组与其他三组之间肠道菌群组成存在差异。与其他组相比，EP+BPH组中[具体菌属名称未给出]的丰度显著增加。与BPH组相比，EP+BPH组中[具体菌属名称未给出]、[具体菌属名称未给出]和[具体菌属名称未给出]显著减少，而与EP组相比，[具体菌属名称未给出]和大肠杆菌显著减少。对于肠道代谢组学，LC-MS/MS显示EP+BPH组中粪便代谢物和七个代谢途径发生了变化，如不饱和脂肪酸的生物合成、类固醇激素的生物合成。相关性分析表明，肠道代谢的改变与肠道菌群差异显著相关，如[具体菌属名称未给出]和[具体菌属名称未给出]。