Duque-Valencia July, Diaz Francisco J, Ruiz-Saenz Julian
Grupo de Investigación en Ciencias Animales-GRICA, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, sede Bucaramanga, Calle 30A # 33-51, 680002 Bucaramanga, Colombia.
Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia, Calle 70 No. 52-21, 050010 Medellín, Colombia.
Pathogens. 2019 Dec 27;9(1):26. doi: 10.3390/pathogens9010026.
Canine distemper virus (CDV) is considered a reemerging disease-causing agent in domestic dogs because it presents high divergence among circulating strains worldwide. In Colombia, the South America-3 and South America/North America-4 lineages co-circulate in domestic dogs, both in the Medellin metropolitan area. In this paper, two full CDV genomes from each viral lineage circulating in Medellin were sequenced; we explored the phylogenetic relationship with the available genome sequences; we described the presence of CDV mutations in the South America-3 and South America/North America-4 lineages associated with adaptation to human cells and a crossing of the species barrier and pathogenicity; and we established the evolutionary rates and time of the closest common ancestor for each gene and characterized the presentation of multiple genomic sites by positive selection.
犬瘟热病毒（CDV）被认为是家犬中一种再度出现的致病因子，因为它在全球流行毒株中呈现出高度差异。在哥伦比亚，南美-3和南美/北美-4谱系在麦德林都会区的家犬中共同传播。在本文中，对麦德林流行的每个病毒谱系的两个完整CDV基因组进行了测序；我们探索了与可用基因组序列的系统发育关系；我们描述了南美-3和南美/北美-4谱系中与适应人类细胞、跨越物种屏障和致病性相关的CDV突变的存在；我们确定了每个基因的进化速率和最近共同祖先的时间，并通过正选择对多个基因组位点的表现进行了表征。
