Department of Bioengineering, Faculty of Engineering, Marmara University, Istanbul, Turkey.
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul, Turkey.
Cartilage. 2021 Dec;13(2_suppl):626S-635S. doi: 10.1177/1947603519897302. Epub 2020 Jan 1.
The aim of this study is to evaluate the mechanical and biological performance of cartilage-like constructs produced by 3D printing. During the investigation, poly(ε-caprolactone) (PCL) and polyvinylpyrrolidone (PVP) were used as a matrix polymer and low-molecular-weight chitosan (CS), hyaluronic acid (HA), and alginic acid sodium salt (SA) were integrated separately with the polymer matrix to fabricate the constructs. Thermal, mechanical, morphology, and chemical properties and swelling, degradation, and biocompatibility behaviors were evaluated in detail. With the addition of 3 fillers, the melting temperature of the matrix increased with the addition of fillers, and PCL/3wt.%PVP/1wt.%HA had the highest melting temperature value. Mechanical characterization results demonstrated that the printed PCL/3wt.%PVP/1wt.%CS displayed the highest compressive strength of around 9.51 MPa. The compressive strength difference between the PCL/3wt.%PVP and PCL/3wt.%PVP/1wt.%CS was 5.38 MPa. Biocompatibility properties of the constructs were tested by mitochondrial dehydrogenase activity, and studies showed that the PCL/3wt.%PVP/1wt.%HA composite construct had more cell viability than the other constructs by making use of the mesenchymal stem cell line.
本研究旨在评估通过 3D 打印制造的类软骨结构的机械和生物学性能。研究过程中，使用聚己内酯（PCL）和聚乙烯吡咯烷酮（PVP）作为基质聚合物，分别将低分子量壳聚糖（CS）、透明质酸（HA）和海藻酸钠（SA）与聚合物基质复合，以制备结构物。详细评估了其热学、力学、形态、化学性能以及溶胀、降解和生物相容性行为。随着 3 种填充剂的加入，基质的熔融温度随填充剂的增加而升高，而 PCL/3wt.%PVP/1wt.%HA 的熔融温度值最高。力学特性结果表明，打印的 PCL/3wt.%PVP/1wt.%CS 具有约 9.51 MPa 的最高抗压强度。PCL/3wt.%PVP 和 PCL/3wt.%PVP/1wt.%CS 之间的抗压强度差异为 5.38 MPa。通过使用间充质干细胞系，通过线粒体脱氢酶活性测试了结构物的生物相容性特性，研究表明，PCL/3wt.%PVP/1wt.%HA 复合材料结构具有比其他结构更高的细胞活力。
