Key Laboratory for Micro/Nano Technology and System of Liaoning Province, Dalian University of Technology, Dalian, 116024, China.
Key Laboratory for Micro/Nano Technology and System of Liaoning Province, Dalian University of Technology, Dalian, 116024, China; Key Laboratory for Precision and Non-traditional Machining Technology of Ministry of Education, Dalian University of Technology, Dalian, 116024, China.
Talanta. 2020 May 1;211:120750. doi: 10.1016/j.talanta.2020.120750. Epub 2020 Jan 16.
Controlled printing of biodegradable and bioresorbable polymers at desired 3D scaffold is of great importance for cell growth and tissue regeneration. In this work, a novel electrohydrodynamic jet 3D printing technology with the resultant effect of electrohydrodynamic force and thermal convection was developed, and its feasibility to fabricate controllable filament composite scaffolds was verified. This method introduces an effective thermal field under the needle to simultaneously enhance the ink viscosity, jetting morphology controllability and printing structure solidify. The fabrication mechanisms of thermal convection on jetting morphology and printed structures feature were investigated through theoretical analysis and experimental characterization. Under optimized conditions, a stable and finer jet was formed; then with the use of this jet, various 3D structures were directly printed at a high aspect ratio 30. Furthermore, the PCL/PVP composite scaffolds with the controllable filament diameter (10 μm) which is closed to living cells were printed. Cell culture experiments showed that the printed scaffolds had excellent cell biocompatibility and facilitated cellular proliferation in vitro. It is a great potential that the developed electrohydrodynamic jet 3D printing technology might provide a novel approach to directly print composite synthetic biopolymers into flexibly scale structures for tissue engineering applications.
在所需的 3D 支架中控制可生物降解和可生物吸收聚合物的打印对于细胞生长和组织再生非常重要。在这项工作中，开发了一种新颖的电动力学射流 3D 打印技术，其具有电动力学力和热对流的综合效果，并验证了其制造可控长丝复合支架的可行性。该方法在针下引入了有效的热场，同时提高了墨水的粘度、射流形态的可控性和打印结构的固化性。通过理论分析和实验表征研究了热对流对射流形态和打印结构特征的影响。在优化条件下，形成了稳定且更细的射流；然后，使用该射流，可以在高纵横比30 下直接打印各种 3D 结构。此外，还打印了具有可控制的长丝直径（10μm）的 PCL/PVP 复合支架，接近活细胞。细胞培养实验表明，打印的支架具有优异的细胞生物相容性，并促进了体外细胞增殖。这种开发的电动力学射流 3D 打印技术具有很大的潜力，可以为直接将复合合成生物聚合物打印成用于组织工程应用的灵活比例结构提供一种新方法。
