Department of Microbiology, Radboud Universiteit, Nijmegen, The Netherlands.
Leibniz Institute DSMZ, Brunswick, Germany.
Antonie Van Leeuwenhoek. 2020 Dec;113(12):1811-1822. doi: 10.1007/s10482-019-01377-2. Epub 2020 Jan 1.
Aquatic bacteria belonging to the deep-branching phylum Planctomycetes play a major role in global carbon and nitrogen cycles. However, their uncommon morphology and physiology, and their roles and survival on biotic surfaces in marine environments, are only partially understood. Access to axenic cultures of different planctomycetal genera is key to study their complex lifestyles, uncommon cell biology and primary and secondary metabolism in more detail. Here, we describe the characterisation of strain Enr8 isolated from a marine biotic surface in the seawater close to the shallow-sea hydrothermal vent system off Panarea Island, an area with high temperature and pH gradients, and high availability of different sulphur and nitrogen sources resulting in a great microbial diversity. Strain Enr8 showed typical planctomycetal traits such as division by polar budding, aggregate formation and presence of fimbriae and crateriform structures. Growth was observed at ranges of 15-33 °C (optimum 30 °C), pH 6.0-8.0 (optimum 7.0) and at NaCl concentrations from 100 to 1200 mM (optimum 350-700 mM). Strain Enr8 forms white colonies on solid medium and white flakes in liquid culture. Its genome has a size of 6.20 Mb and a G + C content of 59.2%. Phylogenetically, the strain belongs to the genus Blastopirellula. We propose the name Blastopirellula retiformator sp. nov. for the novel species, represented by the type strain Enr8 (DSM 100415 = LMG 29081).
属于深分枝门盘状菌的水生细菌在全球碳氮循环中起着重要作用。然而，它们不常见的形态和生理学，以及它们在海洋环境中生物表面的作用和生存方式，只是部分被了解。获得不同盘状菌属的无菌培养物是研究它们复杂生活方式、不常见的细胞生物学以及初级和次级代谢的关键。在这里，我们描述了从潘那雷阿岛近海浅海热液喷口系统附近的海洋生物表面分离得到的菌株 Enr8 的特征，该地区具有高温和 pH 梯度，以及不同硫和氮源的高可用性，导致微生物多样性很大。菌株 Enr8 表现出典型的盘状菌特征，如极性出芽分裂、聚集形成以及纤毛和坑状结构的存在。其生长范围为 15-33°C（最佳 30°C）、pH 6.0-8.0（最佳 7.0）和 NaCl 浓度为 100-1200 mM（最佳 350-700 mM）。菌株 Enr8 在固体培养基上形成白色菌落，在液体培养中形成白色薄片。其基因组大小为 6.20 Mb，G+C 含量为 59.2%。系统发育上，该菌株属于 Blastopirellula 属。我们建议将新型物种命名为 Blastopirellula retiformator sp. nov.，代表菌株为 Enr8（DSM 100415=LMG 29081）。
