Microbial Drugs Department, Helmholtz-Centre for Infection Research, Brunswick, Germany.
German Centre for Infection Research, Partner Site Hannover-Braunschweig, Brunswick, Germany.
Antonie Van Leeuwenhoek. 2020 Dec;113(12):1953-1963. doi: 10.1007/s10482-020-01456-9. Epub 2020 Aug 14.
Species belonging to the bacterial phylum Planctomycetes are ubiquitous members of the microbial communities in aquatic environments and are frequently isolated from various biotic and abiotic surfaces in marine and limnic water bodies. Planctomycetes have large genomes of up to 12.4 Mb, follow complex lifestyles and display an uncommon cell biology; features which motivate the investigation of members of this phylum in greater detail. As a contribution to the current collection of axenic cultures of Planctomycetes, we here describe strain Pla52 isolated from wood particles in the Baltic Sea. Phylogenetic analysis places the strain in the family Pirellulaceae and suggests two species of the recently described genus Stieleria as current closest neighbours. Strain Pla52n shows typical features of members of the class Planctomycetia, including division by polar budding and the presence of crateriform structures. Colonies of strain Pla52n have a light orange colour, which is an unusual pigmentation compared to the majority of members in the phylum, which show either a pink to red pigmentation or entirely lack pigmentation. Optimal growth of strain Pla52n at 33 °C and pH 7.5 indicates a mesophilic (i.e. with optimal growth between 20 and 45 °C) and neutrophilic growth profile. The strain is an aerobic heterotroph with motile daughter cells. Its genome has a size of 9.6 Mb and a G + C content of 56.0%. Polyphasic analyses justify delineation of the strain from described species within the genus Stieleria. Therefore, we conclude that strain Pla52n = LMG 29463 = VKM B-3447 should be classified as the type strain of a novel species, for which we propose the name Stieleria varia sp. nov.
属于浮霉状菌门（Planctomycetes）的物种是水生环境中微生物群落的普遍成员，并且经常从海洋和淡水水体的各种生物和非生物表面分离出来。浮霉状菌具有高达 12.4 Mb 的大型基因组，遵循复杂的生活方式并表现出不常见的细胞生物学特征；这些特征促使人们更详细地研究该门的成员。作为对当前浮霉状菌属纯培养物集合的贡献，我们在这里描述了从波罗的海木块中分离出的菌株 Pla52。系统发育分析将该菌株置于 Pirellulaceae 科中，并表明最近描述的 Stieleria 属的两个物种是当前最接近的近缘种。菌株 Pla52n 显示出典型的类浮霉状菌（Planctomycetia）成员的特征，包括极性出芽分裂和坑状结构的存在。菌株 Pla52n 的菌落呈浅橙色，与该门大多数成员的粉红色到红色色素沉着或完全缺乏色素沉着相比，这是一种不寻常的色素沉着。菌株 Pla52n 在 33°C 和 pH 7.5 下的最佳生长表明其为嗜中温（即最佳生长温度在 20 到 45°C 之间）和嗜中性生长。该菌株是需氧异养的，具有游动的子细胞。其基因组大小为 9.6 Mb，G+C 含量为 56.0%。多相分析证明了该菌株与 Stieleria 属中描述的种的区别。因此，我们得出结论，菌株 Pla52n = LMG 29463 = VKM B-3447 应被归类为新型物种的模式菌株，我们建议将其命名为 Stieleria varia sp. nov.。
