State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
J Appl Microbiol. 2020 May;128(5):1312-1323. doi: 10.1111/jam.14571. Epub 2020 Jan 17.
To investigate the changes in fermentation quality of whole-plant corn silage ensiled with varying dosages of mixed organic acid salts (MS), and link these dosage changes to shifts in bacterial composition.
Fermentation quality and bacterial community of corn silage ensiled with MS at four application rates (0, 0·67 g kg , 1·33 g kg , and 2·00 g kg ) were investigated. The MS consisted of 40% potassium sorbate and 60% sodium benzoate. The corn silages were conserved for 42 days. Dry matter losses and ammonia nitrogen concentration were linearly declined by up to 19·10 and 33·3% with increasing dosages of MS. MS treatments significantly reduced the pH of silage vs the control group. Further analysis indicated that the improvement of fermentation quality was the result of shifts in bacterial composition, the relative abundance of Lactobacillus paralimentarius and Pediococcus spp. increased while Lactobacillus reuteri, Lactobacillus coryniformis and Klebsiella declined with increasing dosages of MS.
MS effectively improved the fermentation quality of whole-plant corn silage with an optimum dosage of 2·00 g kg .
The correlation between bacterial taxa and fermentation quality provides a potential for the development of novel silage inoculants and for the application of MS on controlling Klebsiella mastitis of livestock farms.
研究不同剂量混合有机酸盐（MS）青贮全株玉米青贮发酵品质的变化，并将这些剂量变化与细菌组成的变化联系起来。
研究了 MS 在四个施用量（0、0·67 g kg、1·33 g kg和 2·00 g kg）下青贮玉米的发酵品质和细菌群落。MS 由 40%山梨酸钾和 60%苯甲酸钠组成。玉米青贮分别青贮 42 天。随着 MS 用量的增加，干物质损失和氨态氮浓度分别线性下降了 19.10%和 33.3%。MS 处理组与对照组相比，青贮的 pH 值显著降低。进一步分析表明，发酵品质的改善是细菌组成变化的结果，随着 MS 用量的增加，Lactobacillus paralimentarius 和 Pediococcus spp 的相对丰度增加，而 Lactobacillus reuteri、Lactobacillus coryniformis 和 Klebsiella 的相对丰度下降。
MS 可有效提高全株玉米青贮的发酵品质，最佳用量为 2·00 g kg。
细菌分类群与发酵品质之间的相关性为开发新型青贮接种剂和应用 MS 控制家畜场克雷伯氏菌乳腺炎提供了潜力。
