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外源乳酸菌和玉米粉对青贮饲料发酵品质及微生物群落的影响

Effects of exogenous lactic acid bacteria and maize meal on fermentation quality and microbial community of silage.

作者信息

Zheng Mingli, Mao Peichun, Tian Xiaoxia, Meng Lin

机构信息

Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

出版信息

Front Microbiol. 2023 Sep 21;14:1276493. doi: 10.3389/fmicb.2023.1276493. eCollection 2023.

DOI:10.3389/fmicb.2023.1276493
PMID:37808300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10551169/
Abstract

is a local Brassicaceae in China, while most of it is directly mowed and discarded after the ornamental period. In order to develop forage resources, this study firstly evaluated the potential preservation of silage. was harvested at full-bloom stage, and ensiled without (CK) or with maize meal (Y5), lactic acid bacteria inoculant (Z) and compound additive (Y5Z) for 60 d. Results of chemical and microbiological analysis showed that a large amount of lactic acid was produced and the final pH value was below 4.1 in silages regardless of additive application. CK silage was well preserved as indicated by the low levels of dry matter loss and butyric acid content, and the predominant genus were identified as and . Y5 silage had potential health risks for humans and animals as seen by frequent occurrence of pathogenic bacteria and . Z and Y5Z silages were poorly preserved, resulting in great dry matter loss and butyric acid content. Considering the abundant acetic acid production, the dominant might possess a heterofermentative pathway in Z and Y5Z silages. In conclusion, has the potential to be long stored as silage because of its sufficient water-soluble carbohydrates, while exogenous lactic acid bacteria and maize meal generally provided little positive effect. In future research, efficient homofermentative strains were suggested to be screened to further enhance the ensiling process of silage.

摘要

是中国当地的十字花科植物,而大部分在观赏期过后直接被割掉丢弃。为了开发饲料资源,本研究首先评估了其青贮的潜在保存效果。在盛花期收获,不添加(CK)或添加玉米粉(Y5)、乳酸菌接种剂(Z)和复合添加剂(Y5Z)青贮60天。化学和微生物分析结果表明,无论是否添加添加剂,青贮饲料中都产生了大量乳酸,最终pH值低于4.1。CK青贮饲料保存良好,干物质损失和丁酸含量较低,优势菌属被鉴定为 和 。Y5青贮饲料对人和动物有潜在健康风险,因为病原菌 和 频繁出现。Z和Y5Z青贮饲料保存不佳,导致干物质大量损失和丁酸含量升高。考虑到乙酸产量丰富,Z和Y5Z青贮饲料中的优势 可能具有异型发酵途径。总之,由于其水溶性碳水化合物充足,有作为青贮饲料长期储存的潜力,而外源乳酸菌和玉米粉通常没有什么积极作用。在未来的研究中,建议筛选高效的同型发酵 菌株,以进一步提高 青贮饲料的青贮过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/59aea2a4db48/fmicb-14-1276493-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/2f607fc63752/fmicb-14-1276493-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/89150fcba91a/fmicb-14-1276493-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/ccd2dfe336f1/fmicb-14-1276493-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/59aea2a4db48/fmicb-14-1276493-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/2f607fc63752/fmicb-14-1276493-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/89150fcba91a/fmicb-14-1276493-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/ccd2dfe336f1/fmicb-14-1276493-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e88/10551169/59aea2a4db48/fmicb-14-1276493-g003.jpg

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