Animal Physiology Laboratory, Department of Life Sciences, University of Sfax, Sciences Faculty of Sfax, Sfax, Tunisia.
Ecology, Biology and physiology laboratory of aquatic organisms, Department of Biological Sciences, Sciences Faculty of Tunis, University Tunis El Manar, 2092, Tunis, Tunisia.
Environ Sci Pollut Res Int. 2020 Mar;27(8):8091-8102. doi: 10.1007/s11356-019-07175-7. Epub 2020 Jan 2.
Zinc is one of the important essential trace minerals to human health due to its antioxidant properties. The present study was conducted to elucidate its potential protective role against maneb-induced nephrotoxicity. For this purpose, animals were randomly divided into four groups of six each. Mice of group I (negative controls) have received daily 0.5 ml of distilled water, a solvent of maneb. Mice of group II (MB) have received 30 mg/kg bw of maneb daily by intraperitoneal way. Mice of group III (MB + Zn) have received the same dose of maneb as group II, along with ZnSO (30 mg/kg bw) daily. Mice of group IV (Zn), considered as positive controls, have received the same dose of ZnSO as group III daily. Our results revealed that ZnSO co-administration to maneb-treated mice decreased kidney levels of malondialdehyde, hydrogen peroxide, protein carbonyls, and advanced oxidation protein products; the levels of non-enzymatic antioxidants like vitamin C, glutathione, and metallothionein. It recovered the alteration of antioxidant enzyme activities (catalase, superoxide dismutase, and glutathione peroxidase) and attenuated DNA fragmentation. Furthermore, this essential trace element was also able to alleviate kidney biomarkers' alterations by lowering plasma levels of creatinine, urea, uric acid, and lactate dehydrogenase. In addition, the histopathological changes induced by maneb were improved following zinc administration. Our results indicated that zinc might be beneficial against maneb-induced renal oxidative damage in mice.
锌是人体健康所必需的微量元素之一，具有抗氧化特性。本研究旨在阐明其对代森锰诱导的肾毒性的潜在保护作用。为此，将动物随机分为四组，每组 6 只。第 I 组（阴性对照）的小鼠每天接受 0.5ml 蒸馏水，这是代森锰的溶剂。第 II 组（MB）的小鼠每天通过腹腔注射 30mg/kg bw 的代森锰。第 III 组（MB + Zn）的小鼠每天接受与第 II 组相同剂量的代森锰，同时每天给予 ZnSO（30mg/kg bw）。第 IV 组（Zn）被认为是阳性对照，每天给予与第 III 组相同剂量的 ZnSO。我们的结果表明，ZnSO 与代森锰处理的小鼠共同给药可降低肾脏中丙二醛、过氧化氢、蛋白质羰基和高级氧化蛋白产物的水平；非酶抗氧化剂如维生素 C、谷胱甘肽和金属硫蛋白的水平。它恢复了抗氧化酶活性（过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶）的改变，并减轻了 DNA 片段化。此外，这种必需微量元素还可以通过降低血浆肌酐、尿素、尿酸和乳酸脱氢酶水平来减轻肾脏生物标志物的改变。此外，锌给药后可改善代森锰引起的组织病理学变化。我们的结果表明，锌可能对小鼠代森锰诱导的肾氧化损伤有益。
